Characterization of T-type calcium current and its contribution to electrical activity in rabbit urethra.

Ursula Anderson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Rabbit urethral smooth muscle cells were studied at 37 degrees C by using the amphotericin B perforated-patch configuration of the patch-clamp technique, using Cs(+)-rich pipette solutions. Two components of current, with electrophysiological and pharmacological properties typical of T- and L-type Ca(2+) currents, were recorded. Fitting steady-state inactivation curves for the L current with a Boltzmann equation yielded a V(1/2) of -41 +/- 3 mV. In contrast, the T current inactivated with a V(1/2) of -76 +/- 2 mV. The L currents were reduced by nifedipine (IC(50) = 225 +/- 84 nM), Ni(2+) (IC(50) = 324 +/- 74 microM), and mibefradil (IC(50) = 2.6 +/- 1.1 microM) but were enhanced when external Ca(2+) was substituted with Ba(2+). The T current was little affected by nifedipine at concentrations
Original languageEnglish
Pages (from-to)C1078-C1088
Number of pages11
JournalAmerican journal of physiology. Cell physiology
Volume286(5)
Issue number5 55-5
DOIs
Publication statusPublished - May 2004

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Urethra
Nifedipine
Mibefradil
Rabbits
Calcium
Boltzmann equation
Clamping devices
Amphotericin B
Patch-Clamp Techniques
Smooth Muscle Myocytes
Muscle
Cells
Pharmacology

Cite this

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title = "Characterization of T-type calcium current and its contribution to electrical activity in rabbit urethra.",
abstract = "Rabbit urethral smooth muscle cells were studied at 37 degrees C by using the amphotericin B perforated-patch configuration of the patch-clamp technique, using Cs(+)-rich pipette solutions. Two components of current, with electrophysiological and pharmacological properties typical of T- and L-type Ca(2+) currents, were recorded. Fitting steady-state inactivation curves for the L current with a Boltzmann equation yielded a V(1/2) of -41 +/- 3 mV. In contrast, the T current inactivated with a V(1/2) of -76 +/- 2 mV. The L currents were reduced by nifedipine (IC(50) = 225 +/- 84 nM), Ni(2+) (IC(50) = 324 +/- 74 microM), and mibefradil (IC(50) = 2.6 +/- 1.1 microM) but were enhanced when external Ca(2+) was substituted with Ba(2+). The T current was little affected by nifedipine at concentrations",
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Characterization of T-type calcium current and its contribution to electrical activity in rabbit urethra. / Anderson, Ursula.

In: American journal of physiology. Cell physiology, Vol. 286(5), No. 5 55-5, 05.2004, p. C1078-C1088.

Research output: Contribution to journalArticle

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AU - Anderson, Ursula

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