Chick Amniogenisis and Actin

Nuala Tipping, David Wilson

Research output: Contribution to conferenceAbstract

Abstract

Chick amniogenesis has been described, but less is known of the
forces that may drive the development and closure of the amnion.
Actin cable formation is believed to be one of the mechanisms
involved to explain the fusion of tissue, such as dorsal closure in
the Drosophila embryo. This study examined the possible involvement
of an actin cable during closure of the amniotic sac. Chick
amnion development was studied from Stage 10 through to Stage
18 when amnion closure is completed. This study was carried out
under the provisions of the Scientific Procedures on Animal (1986)
Act (United Kingdom) from which embryonic forms are exempt
until the halfway point of gestation, for chick embryos this is
10.5 days. Staining of the developing amnion with FITC-phalloidin
indicated the presence of an actin cable during amniogenesis and
was evident at the closure of the amnion at Stage 17/18. The actin
cable at various stages of amnion development was similar to that
described at the leading edge in embryonic epithelial wound healing
and embryonic tissue fusion. Transmission electron microscopy
(TEM) at Stage 14 of the developing amnion, treated with a fixative
to preserve actin, revealed linearly arranged microfilaments
in the elongated cells at the leading edge adjacent to either side
of a ‘nodule’ of cells representing the point of midline fusion. These
microfilaments were approximately 6 nm in diameter. Filopodia
and lamellopodia have been described in epidermal embryonic
wound healing and other examples of embryonic epithelial fusion.
In this study lamellopodia were absent and filopodia were almost
absent at the leading edge cells. TEM showed apoptotic cells and
a mesh of cytoplasmic actin filaments dispersed throughout the
accumulated cells of the nodule. This study supports the hypothesis
that an actin cable is involved in amniogenesis, although it reveals
differences in the ultrastructural morphology from that reported
in other embryonic tissue movements.
Original languageEnglish
Pages576
Number of pages1
DOIs
Publication statusPublished - Jan 2006
EventWinter Meeting of the Anatomical Society of Great Britain and Ireland - St Annes College, Oxford University, Oxford, United Kingdom
Duration: 04 Jan 200606 Jan 2006

Conference

ConferenceWinter Meeting of the Anatomical Society of Great Britain and Ireland
CountryUnited Kingdom
CityOxford
Period04/01/200606/01/2006

Fingerprint

Amnion
Actins
Transmission Electron Microscopy
Pseudopodia
Fluorescein-5-isothiocyanate
Chick Embryo
Cytoskeleton
Actin Cytoskeleton
Drosophila
Embryonic Structures
Staining and Labeling
Pregnancy
Wounds and Injuries

Cite this

Tipping, N., & Wilson, D. (2006). Chick Amniogenisis and Actin. 576. Abstract from Winter Meeting of the Anatomical Society of Great Britain and Ireland, Oxford, United Kingdom. https://doi.org/10.1111/j.1469-7580.2006.00629.x
Tipping, Nuala ; Wilson, David. / Chick Amniogenisis and Actin. Abstract from Winter Meeting of the Anatomical Society of Great Britain and Ireland, Oxford, United Kingdom.1 p.
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Tipping, N & Wilson, D 2006, 'Chick Amniogenisis and Actin', Winter Meeting of the Anatomical Society of Great Britain and Ireland, Oxford, United Kingdom, 04/01/2006 - 06/01/2006 pp. 576. https://doi.org/10.1111/j.1469-7580.2006.00629.x

Chick Amniogenisis and Actin. / Tipping, Nuala; Wilson, David.

2006. 576 Abstract from Winter Meeting of the Anatomical Society of Great Britain and Ireland, Oxford, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Chick Amniogenisis and Actin

AU - Tipping, Nuala

AU - Wilson, David

PY - 2006/1

Y1 - 2006/1

N2 - Chick amniogenesis has been described, but less is known of theforces that may drive the development and closure of the amnion.Actin cable formation is believed to be one of the mechanismsinvolved to explain the fusion of tissue, such as dorsal closure inthe Drosophila embryo. This study examined the possible involvementof an actin cable during closure of the amniotic sac. Chickamnion development was studied from Stage 10 through to Stage18 when amnion closure is completed. This study was carried outunder the provisions of the Scientific Procedures on Animal (1986)Act (United Kingdom) from which embryonic forms are exemptuntil the halfway point of gestation, for chick embryos this is10.5 days. Staining of the developing amnion with FITC-phalloidinindicated the presence of an actin cable during amniogenesis andwas evident at the closure of the amnion at Stage 17/18. The actincable at various stages of amnion development was similar to thatdescribed at the leading edge in embryonic epithelial wound healingand embryonic tissue fusion. Transmission electron microscopy(TEM) at Stage 14 of the developing amnion, treated with a fixativeto preserve actin, revealed linearly arranged microfilamentsin the elongated cells at the leading edge adjacent to either sideof a ‘nodule’ of cells representing the point of midline fusion. Thesemicrofilaments were approximately 6 nm in diameter. Filopodiaand lamellopodia have been described in epidermal embryonicwound healing and other examples of embryonic epithelial fusion.In this study lamellopodia were absent and filopodia were almostabsent at the leading edge cells. TEM showed apoptotic cells anda mesh of cytoplasmic actin filaments dispersed throughout theaccumulated cells of the nodule. This study supports the hypothesisthat an actin cable is involved in amniogenesis, although it revealsdifferences in the ultrastructural morphology from that reportedin other embryonic tissue movements.

AB - Chick amniogenesis has been described, but less is known of theforces that may drive the development and closure of the amnion.Actin cable formation is believed to be one of the mechanismsinvolved to explain the fusion of tissue, such as dorsal closure inthe Drosophila embryo. This study examined the possible involvementof an actin cable during closure of the amniotic sac. Chickamnion development was studied from Stage 10 through to Stage18 when amnion closure is completed. This study was carried outunder the provisions of the Scientific Procedures on Animal (1986)Act (United Kingdom) from which embryonic forms are exemptuntil the halfway point of gestation, for chick embryos this is10.5 days. Staining of the developing amnion with FITC-phalloidinindicated the presence of an actin cable during amniogenesis andwas evident at the closure of the amnion at Stage 17/18. The actincable at various stages of amnion development was similar to thatdescribed at the leading edge in embryonic epithelial wound healingand embryonic tissue fusion. Transmission electron microscopy(TEM) at Stage 14 of the developing amnion, treated with a fixativeto preserve actin, revealed linearly arranged microfilamentsin the elongated cells at the leading edge adjacent to either sideof a ‘nodule’ of cells representing the point of midline fusion. Thesemicrofilaments were approximately 6 nm in diameter. Filopodiaand lamellopodia have been described in epidermal embryonicwound healing and other examples of embryonic epithelial fusion.In this study lamellopodia were absent and filopodia were almostabsent at the leading edge cells. TEM showed apoptotic cells anda mesh of cytoplasmic actin filaments dispersed throughout theaccumulated cells of the nodule. This study supports the hypothesisthat an actin cable is involved in amniogenesis, although it revealsdifferences in the ultrastructural morphology from that reportedin other embryonic tissue movements.

U2 - 10.1111/j.1469-7580.2006.00629.x

DO - 10.1111/j.1469-7580.2006.00629.x

M3 - Abstract

SP - 576

ER -

Tipping N, Wilson D. Chick Amniogenisis and Actin. 2006. Abstract from Winter Meeting of the Anatomical Society of Great Britain and Ireland, Oxford, United Kingdom. https://doi.org/10.1111/j.1469-7580.2006.00629.x