Citrate synthase from the liver fluke Fasciola hepatica

Veronika L Zinsser, Catherine M Moore, Elizabeth M Hoey, Alan Trudgett, David J Timson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Citrate synthase catalyses the first step of the Krebs' tricarboxylic acid cycle. A sequence encoding citrate synthase from the common liver fluke, Fasciola hepatica, has been cloned. The encoded protein sequence is predicted to fold into a largely a-helical protein with high structural similarity to mammalian citrate synthases. Although a hexahistidine-tagged version of the protein could be expressed in Escherichia coli, it was not possible to purify it by nickel-affinity chromatography. Similar results were obtained with a version of the protein which lacks the putative mitochondrial targeting sequence (residues 1 to 29). However, extracts from bacterial cells expressing this version had additional citrate synthase activity after correcting for the endogenous, bacterial activity. The apparent K m for oxaloacetate was found to be 0.22 mM, which is higher than that observed in mammalian citrate synthases. Overall, the sequence and structure of F. hepatica citrate synthase are similar to ones from other eukaryotes, but there are enzymological differences which merit further investigation.
Original languageEnglish
Pages (from-to)2413-2417
JournalParasitology Research
Volume112
Issue number6
Early online date14 Mar 2013
DOIs
Publication statusPublished - Jun 2013

Fingerprint

liver flukes
Citrate (si)-Synthase
Fasciola hepatica
citrate (si)-synthase
His-His-His-His-His-His
Proteins
Oxaloacetic Acid
Citric Acid Cycle
proteins
tricarboxylic acid cycle
affinity chromatography
Cell Extracts
Nickel
Eukaryota
nickel
Affinity Chromatography
microbial activity
eukaryotic cells
amino acid sequences
Escherichia coli

Cite this

Zinsser, V. L., Moore, C. M., Hoey, E. M., Trudgett, A., & Timson, D. J. (2013). Citrate synthase from the liver fluke Fasciola hepatica. Parasitology Research, 112(6), 2413-2417. https://doi.org/10.1007/s00436-013-3363-x
Zinsser, Veronika L ; Moore, Catherine M ; Hoey, Elizabeth M ; Trudgett, Alan ; Timson, David J. / Citrate synthase from the liver fluke Fasciola hepatica. In: Parasitology Research. 2013 ; Vol. 112, No. 6. pp. 2413-2417.
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Zinsser, VL, Moore, CM, Hoey, EM, Trudgett, A & Timson, DJ 2013, 'Citrate synthase from the liver fluke Fasciola hepatica', Parasitology Research, vol. 112, no. 6, pp. 2413-2417. https://doi.org/10.1007/s00436-013-3363-x

Citrate synthase from the liver fluke Fasciola hepatica. / Zinsser, Veronika L; Moore, Catherine M; Hoey, Elizabeth M; Trudgett, Alan; Timson, David J.

In: Parasitology Research, Vol. 112, No. 6, 06.2013, p. 2413-2417.

Research output: Contribution to journalArticle

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Zinsser VL, Moore CM, Hoey EM, Trudgett A, Timson DJ. Citrate synthase from the liver fluke Fasciola hepatica. Parasitology Research. 2013 Jun;112(6):2413-2417. https://doi.org/10.1007/s00436-013-3363-x