Cloning and characterization of the Burkholderia vietnamiensis norM gene encoding a multi-drug efflux protein

Christine C Fehlner-Gardiner, Miguel A Valvano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Polymyxin B-sensitive mutants in Burkholderia vietnamiensis (Burkholderia cepacia genomovar V) were generated with a mini-Tn5 encoding tetracycline resistance. One of the transposon mutants had an insertion in the norM gene encoding a multi-drug efflux protein. Expression of B. vietnamiensis norM in an Escherichia coli acrAB deletion mutant complemented its norfloxacin hypersensitivity, indicating that the protein functions in drug efflux. However, no effect on antibiotic sensitivity other than sensitivity to polymyxin B was observed in the B. vietnamiensis norM mutant. We demonstrate that increased polymyxin sensitivity in B. vietnamiensis was associated with the presence of tetracycline in the growth medium, a phenotype that was partially suppressed by expression of the norM gene.
Original languageEnglish
Pages (from-to)279-83
Number of pages5
JournalFEMS Microbiology Letters
Volume215
Issue number2
Publication statusPublished - 08 Oct 2002

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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