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The effect of a cold (<40 °C) radio frequency-driven atmospheric pressure plasma jet on plasmid DNA has been investigated. Gel electrophoresis was used to analyze the DNA forms post-treatment. The experimental data are fitted to a rate equation model that allows for quantitative determination of the rates of single and double strand break formation. The formation of double strand breaks correlates well with the atomic oxygen density. Taken with other measurements, this indicates that neutral components in the jet are effective in inducing double strand breaks.
Bibliographical noteThe authors would like to acknowledge support from UK EPSRC through a Career Acceleration Fellowship (EP/H003797/1) and a Science and Innovation Award (EP/D06337X/1), and the Department of Employment and Learning, Northern Ireland.
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
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