Combined fluorescent in situ hybridisation and immunolabelling of Bacteroides fragilis

G Ramage, S Patrick, S Houston

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We have demonstrated that pure cultures of Bacteroides fragilis can be riboprobed with the oligoprobes BAC303 and EUB338, whilst simultaneously immunolabelled with either the mAb QUBF7, or polyclonal antiserum specific for a common antigen of B. fragilis. We were also able to distinguish between pure cultures of B. fragilis and Escherichia coli, by means of combined immunolabelling and riboprobing. The success of the combined technique is critically dependent on the size of the bacterial capsules, bacterial growth phase, antibody diluent and the length of the washing steps. The combined FISH and immunolabelling of bacteria has potential applications in studies of bacteria of medical and veterinary importance, as well as bacteria from other environments, as it yields information about both the identity and antigen expression of individual bacterial cells.
Original languageEnglish
Pages (from-to)139-47
Number of pages9
JournalJournal of Immunological Methods
Volume212
Issue number2
Publication statusPublished - 1998

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