COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks. COMMD4 regulates chromatin at DNA double-strand breaks

Amila Suraweera, Neha Gandhi, Beard Sam , Burgess Joshua , Laura V. Croft, Emma Bolderson, Ali Naqi, Nicholas W. Ashton, Mark Adams, Kienan Savage, Shu‐Dong Zhang, Kenneth J O'Byrne, Derek J. Richard

Research output: Contribution to journalArticlepeer-review

Abstract

Genomic stability is critical for normal cellular function and its deregulation is a universal hallmark of cancer. Here we outline a previously undescribed role of COMMD4 in maintaining genomic stability, by regulation of chromatin remodelling at sites of DNA double-strand breaks. At break-sites, COMMD4 binds to and protects histone H2B from monoubiquitination by RNF20/RNF40. DNA damage-induced phosphorylation of the H2A-H2B heterodimer disrupts the dimer allowing COMMD4 to preferentially bind H2A.Displacement of COMMD4 from H2B allows RNF20/40 to monoubiquitinate H2B and for remodelling of the break-site. Consistent with this critical function, COMMD4-deficient cells show excessive elongation of remodelled chromatin and failure of both non-homologous-end-joining and homologous recombination. We present peptide-mapping and mutagenesis data for the potential molecular mechanisms governing COMMD4-mediated chromatin regulation at DNA double-strand breaks.
Original languageEnglish
JournalCommunications Biology
Publication statusAccepted - 18 Mar 2021

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