Comparative analysis of dna methylation profiles in peripheral blood leukocytes versus lymphoblastoid cell lines

Eugene Brennan, M. Ehrich, Derek Brazil, J.K. Crean, M. Murphy, D.M. Sadlier, F. Martin, C. Godson, Amy Jayne McKnight, D. Van Den Boom, Alexander Maxwell, D.A. Savage

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Previous reports have shown that DNA methylation profiles within primary human malignant tissues are altered when these cells are transformed into cancer cell lines. However, it is unclear if similar differences in DNA methylation profiles exist between DNA derived from peripheral blood leukocytes (PBLs) and corresponding Epstein-Barr Virus transformed lymphoblastoid cell lines (LCLs). To assess the utility of LCLs as a resource for methylation studies we have compared DNA methylation profiles in promoter and 5' regions of 318 genes in PBL and LCL sample pairs from patients with type 1 diabetes with or without nephropathy. We identified a total of 27 (similar to 8%) genes that revealed different DNA methylation profiles in PBL compared with LCL-derived DNA samples. In conclusion, although the profiles for most promoter regions were similar between PBL-LCL pairs, our results indicate that LCL-derived DNA may not be suitable for DNA methylation studies at least in diabetic nephropathy.
Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalEpigenetics
Volume4
Issue number3
Publication statusPublished - 01 Apr 2009

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Fingerprint Dive into the research topics of 'Comparative analysis of dna methylation profiles in peripheral blood leukocytes versus lymphoblastoid cell lines'. Together they form a unique fingerprint.

  • Cite this

    Brennan, E., Ehrich, M., Brazil, D., Crean, J. K., Murphy, M., Sadlier, D. M., Martin, F., Godson, C., McKnight, A. J., Van Den Boom, D., Maxwell, A., & Savage, D. A. (2009). Comparative analysis of dna methylation profiles in peripheral blood leukocytes versus lymphoblastoid cell lines. Epigenetics, 4(3), 159-164.