Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish

Juan José Dorantes-Aranda, Katrina Campbell, Andrew Bradbury, Christopher T Elliott, D Tim Harwood, Shauna A Murray, Sarah C Ugalde, Katrina Wilson, Megan Burgoyne, Gustaaf M Hallegraeff

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)
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Abstract

Blooms of the toxic dinoflagellate Alexandrium tamarense (Group 1) seriously impacted the Tasmanian shellfish industry during 2012 and 2015, necessitating product recalls and intensive paralytic shellfish toxin (PST) product testing. The performance of four commercial PST test kits, Abraxis™, Europroxima™, Scotia™ and Neogen™, was compared with the official AOAC LC-FLD method for contaminated mussels and oysters. Abraxis and Europroxima kits underestimated PST in 35-100% of samples when using standard protocols but quantification improved when concentrated extracts were further diluted (underestimation ≤18%). The Scotia kit (cut off 0.2-0.7 mg STX-diHCl eq/kg) delivered 0% false negatives, but 27% false positives. Neogen produced 5% false negatives and 13% false positives when the cut off was altered to 0.5-0.6 mg STX-diHCl eq/kg, the introduction of a conversion step eliminated false negatives. Based on their sensitivity, ease of use and performance, the Neogen kit proved the most suitable kit for use with Tasmanian mussels and oysters. Once formally validated for regulatory purposes, the Neogen kit could provide shellfish growers with a rapid tool for harvesting decisions at the farm gate. Effective rapid screening preventing compliant samples undergoing testing using the more expensive and time consuming LC-FLD method will result in significant savings in analytical costs.

Original languageEnglish
Pages (from-to)110-119
Number of pages10
JournalToxicon
Volume125
Early online date30 Nov 2016
DOIs
Publication statusPublished - Jan 2017

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