Comparative Profiling of Three Atheris Snake Venoms: A. squamigera, A. nitschei and A. chlorechis

He Wang, Xiaole Chen, Enrico König, Mei Zhou, Lei Wang, Tianbao Chen, Christopher Shaw

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
987 Downloads (Pure)


A proteomic and transcriptomic comparative analysis of the venoms of three Atheris species (A. squamigera, A. nitschei and A. chlorechis) was carried out by size exclusion liquid chromatography, gel electrophoresis, mass spectrometry, and mRNA sequencing. The improved proteomic profiling utilised in this work was combined with transcript studies, advancing our insights into venom composition, protein distribution and inter-species variation among the three bush vipers. Crude venoms of all three samples contained at least 10-20 protein components, ranging in size from ≤ 3 to > 98 kDa. Both approaches yielded converging overall information, pointing to phospholipases, disintegrins, serine proteases and metalloproteases as the major toxin classes, which are likely to explain the local and systemic symptoms observed in envenomation by Atheris genus. Being considered as the main factors involved in the distinct venom-induced pathologies, these identified snake venom proteins are of particular interest in terms of understanding their physiological and biological function as well as for their contribution in potential medical treatments.
Original languageEnglish
Pages (from-to)353-360
JournalThe Protein Journal
Issue number4
Early online date16 Jul 2018
Publication statusPublished - Aug 2018


  • Atheris; Proteomics; Snake venom; Toxins


Dive into the research topics of 'Comparative Profiling of Three Atheris Snake Venoms: A. squamigera, A. nitschei and A. chlorechis'. Together they form a unique fingerprint.

Cite this