Comparative Proteomics Implicates a Role for Multiple Secretion Systems in Electrode-Respiring Geobacter sulfurreducens Biofilms

Paul Kavanagh*, Catherine H. Botting, Partha S. Jana, Dónal Leech, Florence Abram

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Geobacter sulfurreducens is a dissimilatory metal-reducing bacterium capable of forming thick electron-conducting biofilms on solid electrodes. Here we employ for the first time comparative proteomics to identify key physiological changes involved in G. sulfurreducens adaptation from fumarate-respiring planktonic cells to electron-conducting biofilms. Increased levels of proteins involved in outer membrane biogenesis, cell motility, and secretion are expressed in biofilms. Of particular importance to the electron-conducting biofilms are proteins associated with secretion systems of Type I, II, V and Type IV pili. Furthermore, enzymes involved in lipopolysaccharide and peptidoglycan biosynthesis show increased levels of expression in electron-conducting biofilms compared with planktonic cells. These observations point to similarities in long-range electron-transfer mechanisms between G. sulfurreducens and Shewanella oneidensis while highlighting the wider significance of secretion systems beyond that of Type IV pili identified to date in the adaptation of G. sulfurreducens to electrode respiration.

Original languageEnglish
Pages (from-to)4135-4145
Number of pages11
JournalJournal of Proteome Research
Volume15
Issue number12
Early online date10 Oct 2016
DOIs
Publication statusPublished - 02 Dec 2016

Fingerprint

Geobacter
Biofilms
Proteomics
Electrodes
Electrons
Shewanella
Fumarates
Peptidoglycan
Biosynthesis
Cell membranes
Cell Movement
Lipopolysaccharides
Bacteria
Respiration
Proteins
Metals
Membranes
Enzymes

Keywords

  • electrode biofilm
  • electron transfer
  • Geobacter sulfurreducens
  • proteomics
  • secretions systems

Cite this

Kavanagh, Paul ; Botting, Catherine H. ; Jana, Partha S. ; Leech, Dónal ; Abram, Florence. / Comparative Proteomics Implicates a Role for Multiple Secretion Systems in Electrode-Respiring Geobacter sulfurreducens Biofilms. In: Journal of Proteome Research. 2016 ; Vol. 15, No. 12. pp. 4135-4145.
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Comparative Proteomics Implicates a Role for Multiple Secretion Systems in Electrode-Respiring Geobacter sulfurreducens Biofilms. / Kavanagh, Paul; Botting, Catherine H.; Jana, Partha S.; Leech, Dónal; Abram, Florence.

In: Journal of Proteome Research, Vol. 15, No. 12, 02.12.2016, p. 4135-4145.

Research output: Contribution to journalArticle

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