Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum

L. Moertel, G. N. Gobert, D. P. McManus

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Schistosomes are complex parasitic helminths with discrete life-cycle stages, adapted for survival in their mammalian and snail hosts and the external aquatic environment. Recently, we described the fabrication and use of a microarray to investigate gender-specific transcription in Schistosoma japonicum. To address transcriptional differences, 8 gender-associated gene transcripts identified previously by the microarray analysis were selected for further study. First, differential transcription patterns were investigated in 4 developmental stages using real-time PCR. Subsequently, we undertook functional analysis of a subset of 4 transcripts encoding metabolic enzymes, so as to correlate gender-associated transcript levels with enzyme activity in protein extracts from adult worms. The 8 characterized molecules serve as a basis for further investigation of differential gene expression during the schistosome life-cycle and for studying the sexual dimorphism of adult worms. Continual refinement and annotation of the microarray used in the current study should support future work on these aspects.

Original languageEnglish
Pages (from-to)575-583
Number of pages9
JournalParasitology
Volume135
Issue number5
DOIs
Publication statusPublished - 01 Apr 2008
Externally publishedYes

Keywords

  • Gene expression
  • Microarray
  • Real-time PCR
  • Schistosoma
  • Schistosoma japonicum

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

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