Abstract
Beef longissimus dorsi muscle samples matured over a 21 day period were analysed using three different analytical techniques; 1H NMR, GC-MS and HPLC. The data from the three experimental techniques were correlated with each other to determine if the results were statistically similar to each other. From our analysis we determined that the metabolites measured using 1H NMR were statistically similar to the compounds quantified using the chromatography techniques (p < 0.001). In addition, using PCA, we were able to show that different metabolites, measured using the various analytical techniques produced very similar scores and loadings plots for all the analysis and extraction techniques undertaken across the 21 day time domain. Using a combination of these three different techniques provides a unique and holistic insight into the biochemistry behind the conversion of muscle to meat which would not be possible using any single technique alone.
Original language | English |
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Pages (from-to) | 1633-1639 |
Number of pages | 7 |
Journal | Food Chemistry |
Volume | 134 |
Issue number | 3 |
DOIs | |
Publication status | Published - 01 Oct 2012 |
Keywords
- Beef
- GC-MS
- HPLC
- NMR
- Profiling
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science