Comparison of reporter molecules for viral in situ hybridization

S. McQuaid; G. M. Allan; M. J. Taylor; D. Todd; J. Smith; S. L. Cosby; I. V. Allen

doi:10.1016/0166-0934(91)90140-U

Comparison of reporter molecules for viral in situ hybridization

S. McQuaid^*, G. M. Allan, M. J. Taylor, D. Todd, J. Smith, S. L. Cosby, I. V. Allen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A number of streptavidin-linked reporter molecules at the endpoint of a five-step detection protocol for viral in situ hybridization using biotinylated probes were examined. DNA-DNA and RNA-RNA model systems were used. Streptavidin linked to either peroxidase or fluorescein was found to be optimal in terms of sensitivity and resolution within individual cells. All other reporter molecules labelled similar numbers of cells with low background reaction. However, streptavidin-5 nm gold followed by silver enhancement gave very high background staining making interpretation of positive signals very difficult.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of Virological Methods
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(91)90140-U
Publication status	Published - Jan 1991

Bibliographical note

Funding Information:
This work was supportedi n part by the Multiple Britain and Northern Ireland.

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

Fluorescein
Peroxidase
Reporter molecule
Streptavidin

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(91)90140-U

Cite this

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AU - Allan, G. M.

AU - Taylor, M. J.

AU - Todd, D.

AU - Smith, J.

AU - Cosby, S. L.

AU - Allen, I. V.

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AB - A number of streptavidin-linked reporter molecules at the endpoint of a five-step detection protocol for viral in situ hybridization using biotinylated probes were examined. DNA-DNA and RNA-RNA model systems were used. Streptavidin linked to either peroxidase or fluorescein was found to be optimal in terms of sensitivity and resolution within individual cells. All other reporter molecules labelled similar numbers of cells with low background reaction. However, streptavidin-5 nm gold followed by silver enhancement gave very high background staining making interpretation of positive signals very difficult.

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KW - Peroxidase

KW - Reporter molecule

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Comparison of reporter molecules for viral in situ hybridization

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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