Comparison of sample preparation methods, validation of an UPLC-MS/MS procedure for the quantification of tetrodotoxin present in marine gastropods and analysis of pufferfish

Judith Kouassi Nzoughet, Katrina Campbell, Paul Barnes, Kevin M Cooper, Olivier P Chevallier, Christopher T Elliott

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Tetrodotoxin (TTX) is one of the most potent marine neurotoxins reported. The global distribution of this toxin is spreading with the European Atlantic coastline now being affected. Climate change and increasing pollution have been suggested as underlying causes for this. In the present study, two different sample preparation techniques were used to extract TTX from Trumpet shells and pufferfish samples. Both extraction procedures (accelerated solvent extraction (ASE) and a simple solvent extraction) were shown to provide good recoveries (80-92%). A UPLC-MS/MS method was developed for the analysis of TTX and validated following the guidelines contained in the Commission Decision 2002/657/EC for chemical contaminant analysis. The performance of this procedure was demonstrated to be fit for purpose. This study is the first report on the use of ASE as a mean for TTX extraction, the use of UPLC-MS/MS for TTX analysis, and the validation of this method for TTX in gastropods.
Original languageEnglish
Pages (from-to)1584-9
JournalFood Chemistry
Volume136
Issue number3-4
Early online date08 Feb 2012
DOIs
Publication statusPublished - 15 Feb 2013

Bibliographical note

Copyright © 2012 Elsevier Ltd. All rights reserved.

Keywords

  • Analytic Sample Preparation Methods
  • Animals
  • Chromatography, High Pressure Liquid
  • Gastropoda
  • Tandem Mass Spectrometry
  • Tetraodontiformes
  • Tetrodotoxin

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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