Comparison of single and multi-analyte methods based on LC-MS/MS for mycotoxin biomarker determination in human urine

M. Solfrizzo*, L. Gambacorta, B. Warth, K. White, C. Srey, M. Sulyok, R. Krska, Y. Y. Gong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The performances of four LC-MS/MS methodologies for determination of up to eight mycotoxin biomarkers in human urines were compared by involving three laboratories that analysed common urine samples spiked at two levels of each biomarker. Each laboratory received a calibration solution, spiked urines and the corresponding unspiked urine. The two spiking levels for each biomarker were chosen by considering the levels naturally occurring in human urines and the limits of quantification of the LC-MS/MS methodologies used by the participating laboratories. The results of each laboratory were evaluated for their z-score values. The percentage of satisfactory z-scores (vertical bar z vertical bar 2) were obtained for fumonisin B-1 (7/12 results), ochratoxin A (4/8 results) and alpha-zearalenol (1/8 results). The percentage of satisfactory z-scores for fumonisin B-1 and ochratoxin A increased from 42 to 83% for fumonisin B-1 and from 50 to 62% for ochratoxin A when laboratories 1 and 2 used own calibrants. Factors that could explain the different results obtained for fumonisin B-1 and ochratoxin A with provided and own calibration solutions could not be identified in this study and should be carefully investigated in future studies.

Original languageEnglish
Pages (from-to)355-366
Number of pages12
JournalWorld Mycotoxin Journal
Volume6
Issue number4
DOIs
Publication statusPublished - Nov 2013

Keywords

  • mycotoxins
  • urine
  • biomarkers
  • LC-MS/MS
  • clean-up
  • TANDEM MASS-SPECTROMETRY
  • LIQUID-CHROMATOGRAPHY
  • HUMAN EXPOSURE
  • DIETARY-INTAKE
  • FUMONISIN B-1
  • OCHRATOXIN-A
  • DEOXYNIVALENOL
  • ZEARALENONE
  • AFLATOXIN
  • PERFORMANCE

Fingerprint

Dive into the research topics of 'Comparison of single and multi-analyte methods based on LC-MS/MS for mycotoxin biomarker determination in human urine'. Together they form a unique fingerprint.

Cite this