Comparison of three adjuvants used to produce polyclonal antibodies to veterinary drugs

Terence Fodey, P. Delahaut, C. Charlier, Christopher Elliott

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two commercially available adjuvants, Gerbu LQ 3000 and Montanide ISA 50V, were assessed as potential replacements for Freund's adjuvant by evaluating their efficacy in the production of polyclonal antibodies to veterinary drugs in rabbits. The aim was to find an adjuvant that could produce a similar (or enhanced) immune response in the host animal without the undesirable side effects associated with Freund's complete and incomplete adjuvant. The assessment involved the examination of each injection site and the characterisation of the resultant antibodies with regards to antibody titre and sensitivity. It was found that the rabbits immunised with Gerbu adjuvant produced some of the most sensitive antibodies. However, titres were relatively low and adverse effects at injection sites were relatively common. Montanide adjuvant produced no adverse effects and the related antibodies were found to be of adequate sensitivity when compared to those from rabbits immunised with Freund's. It was concluded that Montanide ISA 50V could be considered as a suitable replacement to Freund's for the production of polyclonal antibodies, to low molecular weight compounds in rabbits. (C) 2007 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume122
Issue number1-2
DOIs
Publication statusPublished - 15 Mar 2008

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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