Concordance of Macular Pigment Measurement Using Customized Heterochromatic Flicker Photometry and Fundus Autofluorescence in Age-Related Macular Degeneration

Kwadwo Owusu Akuffo, Stephen Beatty, Jim Stack, Tunde Peto, Irene Leung, Laura Corcoran, Rebecca Power, John M Nolan

Research output: Contribution to journalArticle

10 Citations (Scopus)
177 Downloads (Pure)

Abstract

PURPOSE: We compared macular pigment (MP) measurements using customized heterochromatic flicker photometry (Macular Metrics Densitometer) and dual-wavelength fundus autofluorescence (Heidelberg Spectralis HRA + OCT MultiColor) in subjects with early age-related macular degeneration (AMD).

METHODS: Macular pigment was measured in 117 subjects with early AMD (age, 44-88 years) using the Densitometer and Spectralis, as part of the Central Retinal Enrichment Supplementation Trial (CREST; ISRCTN13894787). Baseline and 6-month study visits data were used for the analyses. Agreement was investigated at four different retinal eccentricities, graphically and using indices of agreement, including Pearson correlation coefficient (precision), accuracy coefficient, and concordance correlation coefficient (ccc).

RESULTS: Agreement was poor between the Densitometer and Spectralis at all eccentricities, at baseline (e.g., at 0.25° eccentricity, accuracy = 0.63, precision = 0.35, ccc = 0.22) and at 6 months (e.g., at 0.25° eccentricity, accuracy = 0.52, precision = 0.43, ccc = 0.22). Agreement between the two devices was significantly greater for males at 0.5° and 1.0° of eccentricity. At all eccentricities, agreement was unaffected by cataract grade.

CONCLUSIONS: In subjects with early AMD, MP measurements obtained using the Densitometer and Spectralis are not statistically comparable and should not be used interchangeably in either the clinical or research setting. Despite this lack of agreement, statistically significant increases in MP, following 6 months of supplementation with macular carotenoids, were detected with each device, confirming that these devices are capable of measuring change in MP within subjects over time. (http://www.controlled-trials.com number, ISRCTN13894787.).

Original languageEnglish
Pages (from-to)8207-8214
Number of pages8
JournalInvestigative ophthalmology & visual science
Volume56
Issue number13
DOIs
Publication statusPublished - Dec 2015

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Densitometry
  • Female
  • Fluorescein Angiography
  • Fundus Oculi
  • Humans
  • Macular Degeneration
  • Macular Pigment
  • Male
  • Middle Aged
  • Photometry
  • Reproducibility of Results
  • Journal Article
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

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