Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods

H. L. Alakomi*, M. Saarela, A. A. Gorbushina, W. E. Krumbeín, C. McCullagh, P. Robertson, K. Rodenacker

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides

Original languageEnglish
Title of host publicationHeritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006)
EditorsRafael Fort, Monica Alvarez de Buergo, Miguel Gomez-Heras, Carmen Vazquez-Calvo
Place of PublicationLondon
PublisherTaylor and Francis
Pages713-717
Number of pages5
Volume2
ISBN (Print)9780415412728
Publication statusPublished - 01 Jun 2006
EventInternational Conference on Heritage, Weathering and Conservation, HWC 2006 - Madrid, Spain
Duration: 21 Jun 200624 Jun 2006

Conference

ConferenceInternational Conference on Heritage, Weathering and Conservation, HWC 2006
CountrySpain
CityMadrid
Period21/06/200624/06/2006

Fingerprint

Biofilms
Biocides
Evaluation
Biofilm
Algae
Fungi
Refractory materials
Image analysis
Bacteria
Microscopic examination
Minerals
Rocks
Scanning
Testing

Cite this

Alakomi, H. L., Saarela, M., Gorbushina, A. A., Krumbeín, W. E., McCullagh, C., Robertson, P., & Rodenacker, K. (2006). Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. In R. Fort, M. Alvarez de Buergo, M. Gomez-Heras, & C. Vazquez-Calvo (Eds.), Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006) (Vol. 2, pp. 713-717). London: Taylor and Francis.
Alakomi, H. L. ; Saarela, M. ; Gorbushina, A. A. ; Krumbeín, W. E. ; McCullagh, C. ; Robertson, P. ; Rodenacker, K. / Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). editor / Rafael Fort ; Monica Alvarez de Buergo ; Miguel Gomez-Heras ; Carmen Vazquez-Calvo . Vol. 2 London : Taylor and Francis, 2006. pp. 713-717
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abstract = "The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as {"}subaerial biofilms{"}. The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides",
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Alakomi, HL, Saarela, M, Gorbushina, AA, Krumbeín, WE, McCullagh, C, Robertson, P & Rodenacker, K 2006, Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. in R Fort, M Alvarez de Buergo, M Gomez-Heras & C Vazquez-Calvo (eds), Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). vol. 2, Taylor and Francis, London, pp. 713-717, International Conference on Heritage, Weathering and Conservation, HWC 2006, Madrid, Spain, 21/06/2006.

Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. / Alakomi, H. L.; Saarela, M.; Gorbushina, A. A.; Krumbeín, W. E.; McCullagh, C.; Robertson, P.; Rodenacker, K.

Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). ed. / Rafael Fort; Monica Alvarez de Buergo; Miguel Gomez-Heras; Carmen Vazquez-Calvo . Vol. 2 London : Taylor and Francis, 2006. p. 713-717.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods

AU - Alakomi, H. L.

AU - Saarela, M.

AU - Gorbushina, A. A.

AU - Krumbeín, W. E.

AU - McCullagh, C.

AU - Robertson, P.

AU - Rodenacker, K.

PY - 2006/6/1

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N2 - The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides

AB - The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides

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SN - 9780415412728

VL - 2

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EP - 717

BT - Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006)

A2 - Fort, Rafael

A2 - Alvarez de Buergo, Monica

A2 - Gomez-Heras, Miguel

A2 - Vazquez-Calvo , Carmen

PB - Taylor and Francis

CY - London

ER -

Alakomi HL, Saarela M, Gorbushina AA, Krumbeín WE, McCullagh C, Robertson P et al. Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. In Fort R, Alvarez de Buergo M, Gomez-Heras M, Vazquez-Calvo C, editors, Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). Vol. 2. London: Taylor and Francis. 2006. p. 713-717