Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods

H. L. Alakomi; M. Saarela; A. A. Gorbushina; W. E. Krumbeín; C. McCullagh; P. Robertson; K. Rodenacker

Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods

H. L. Alakomi^*, M. Saarela, A. A. Gorbushina, W. E. Krumbeín, C. McCullagh, P. Robertson, K. Rodenacker

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

7 Citations (Scopus)

Abstract

The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides

Original language	English
Title of host publication	Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006)
Editors	Rafael Fort, Monica Alvarez de Buergo, Miguel Gomez-Heras, Carmen Vazquez-Calvo
Place of Publication	London
Publisher	Taylor and Francis
Pages	713-717
Number of pages	5
Volume	2
ISBN (Print)	9780415412728
Publication status	Published - 01 Jun 2006
Event	International Conference on Heritage, Weathering and Conservation, HWC 2006 - Madrid, Spain Duration: 21 Jun 2006 → 24 Jun 2006

Conference

Conference	International Conference on Heritage, Weathering and Conservation, HWC 2006
Country/Territory	Spain
City	Madrid
Period	21/06/2006 → 24/06/2006

ASJC Scopus subject areas

Conservation
Civil and Structural Engineering
Building and Construction
Architecture

Access to Document

https://www.crcpress.com/product/isbn/9780415412728

Cite this

Alakomi, H. L., Saarela, M., Gorbushina, A. A., Krumbeín, W. E., McCullagh, C., Robertson, P., & Rodenacker, K. (2006). Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. In R. Fort, M. Alvarez de Buergo, M. Gomez-Heras, & C. Vazquez-Calvo (Eds.), Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006) (Vol. 2, pp. 713-717). Taylor and Francis. https://www.crcpress.com/product/isbn/9780415412728

Alakomi, H. L. ; Saarela, M. ; Gorbushina, A. A. et al. / Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). editor / Rafael Fort ; Monica Alvarez de Buergo ; Miguel Gomez-Heras ; Carmen Vazquez-Calvo . Vol. 2 London : Taylor and Francis, 2006. pp. 713-717

@inproceedings{2c27315fc60849a282d686974c29449d,

title = "Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods",

abstract = "The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as {"}subaerial biofilms{"}. The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides",

author = "Alakomi, {H. L.} and M. Saarela and Gorbushina, {A. A.} and Krumbe{\'i}n, {W. E.} and C. McCullagh and P. Robertson and K. Rodenacker",

year = "2006",

month = jun,

day = "1",

language = "English",

isbn = "9780415412728",

volume = "2",

pages = "713--717",

editor = "Rafael Fort and {Alvarez de Buergo}, Monica and Miguel Gomez-Heras and {Vazquez-Calvo }, Carmen",

booktitle = "Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006)",

publisher = "Taylor and Francis",

address = "United Kingdom",

note = "International Conference on Heritage, Weathering and Conservation, HWC 2006 ; Conference date: 21-06-2006 Through 24-06-2006",

}

Alakomi, HL, Saarela, M, Gorbushina, AA, Krumbeín, WE, McCullagh, C, Robertson, P & Rodenacker, K 2006, Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. in R Fort, M Alvarez de Buergo, M Gomez-Heras & C Vazquez-Calvo (eds), Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). vol. 2, Taylor and Francis, London, pp. 713-717, International Conference on Heritage, Weathering and Conservation, HWC 2006, Madrid, Spain, 21/06/2006. <https://www.crcpress.com/product/isbn/9780415412728>

Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. / Alakomi, H. L.; Saarela, M.; Gorbushina, A. A. et al.
Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). ed. / Rafael Fort; Monica Alvarez de Buergo; Miguel Gomez-Heras; Carmen Vazquez-Calvo . Vol. 2 London: Taylor and Francis, 2006. p. 713-717.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods

AU - Alakomi, H. L.

AU - Saarela, M.

AU - Gorbushina, A. A.

AU - Krumbeín, W. E.

AU - McCullagh, C.

AU - Robertson, P.

AU - Rodenacker, K.

PY - 2006/6/1

Y1 - 2006/6/1

N2 - The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides

AB - The rock/atmosphere interface is inhabited by a complex microbial community including bacteria, algae and fungi. These communities are prominent biodeterioration agents and remarkably influence the status of stone monuments and buildings. Deeper comprehension of natural biodeterioration processes on stone surfaces has brought about a concept of complex microbial communities referred to as "subaerial biofilms". The practical implications of biofilm formation are that control strategies must be devised both for testing the susceptibility of the organisms within the biofilm and treating the established biofilm. Model multi-species biofilms associated with mineral surfaces that are frequently refractory to conventional treatment have been used as test targets. A combination of scanning microscopy with image analysis was applied along with traditional cultivation methods and fluorescent activity stains. Such a polyphasic approach allowed a comprehensive quantitative evaluation of the biofilm status and development. Effective treatment strategies incorporating chemical and physical agents have been demonstrated to prevent biofilm growth in vitro. Model biofilm growth on inorganic support was significantly reduced by a combination of PDT and biocides

UR - http://www.scopus.com/inward/record.url?scp=60649122041&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:60649122041

SN - 9780415412728

VL - 2

SP - 713

EP - 717

BT - Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006)

A2 - Fort, Rafael

A2 - Alvarez de Buergo, Monica

A2 - Gomez-Heras, Miguel

A2 - Vazquez-Calvo , Carmen

PB - Taylor and Francis

CY - London

T2 - International Conference on Heritage, Weathering and Conservation, HWC 2006

Y2 - 21 June 2006 through 24 June 2006

ER -

Alakomi HL, Saarela M, Gorbushina AA, Krumbeín WE, McCullagh C, Robertson P et al. Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods. In Fort R, Alvarez de Buergo M, Gomez-Heras M, Vazquez-Calvo C, editors, Heritage, Weathering and Conservation: Proceedings of the International Heritage, Weathering and Conservation Conference (HWC-2006). Vol. 2. London: Taylor and Francis. 2006. p. 713-717

Control of biofilm growth through photodynamic treatments combined with chemical inhibitors: in vitro evaluation methods

Abstract

Conference

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this