Control of the AtMAP65-1 interaction with microtubules through the cell cycle

Andrei P. Smertenko, Hsin-Yu Chang, Seiji Sonobe, Stepan I. Fenyk, Magdalena Weingartner, Laci Bogre, Patrick J. Hussey*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

Cell division depends on the fine control of both microtubule dynamics and microtubule organisation. The microtubule bundling protein MAP65 is a 'midzone MAP' essential for the integrity of the anaphase spindle and cell division. Arabidopsis thaliana MAP65-1 (AtMAP65-1) binds and bundles microtubules by forming 25 nm cross-bridges. Moreover, as AtMAP65-1 bundles microtubules in interphase, anaphase and telophase but does not bind microtubules in prophase or metaphase, its activity through the cell cycle must be under tight control. Here we show that AtMAP65-1 is hyperphosphorylated during prometaphase and metaphase and that CDK and MAPK are involved in this phosphorylation. This phosphorylation inhibits AtMAP65-1 activity. Expression of nonphosphorylatable AtMAP65-1 has a negative effect on mitotic progression resulting in excessive accumulation of microtubules in the metaphase spindle midzone causing a delay in mitosis. We conclude that normal metaphase spindle organisation and the transition to anaphase is dependent on inactivation of AtMAP65-1.

Original languageEnglish
Pages (from-to)3227-3237
Number of pages11
JournalJournal of Cell Science
Volume119
Issue number15
DOIs
Publication statusPublished - 01 Aug 2006

Keywords

  • division midzone
  • PLANT CYTOKINESIS
  • MITOTIC SPINDLE
  • PRC1
  • KINESIN-RELATED PROTEIN
  • MIDZONE FORMATION
  • IN-VIVO
  • microtubule-associated protein
  • PLATE FORMATION
  • PHRAGMOPLAST
  • ACTIVATED PROTEIN-KINASE
  • MAP KINASE
  • regulation
  • MAP-65

Cite this