Cotranscriptional role for Arabidopsis DICER-LIKE 4 in transcription termination.

Fuquan Liu, Saleha Bakht, Caroline Dean

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference–mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.
Original languageEnglish
Pages (from-to)1621-1623
Number of pages3
JournalScience
Volume335
Issue number6076
DOIs
Publication statusPublished - 30 Mar 2012

ASJC Scopus subject areas

  • General
  • History and Philosophy of Science

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