Abstract
Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference–mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.
| Original language | English |
|---|---|
| Pages (from-to) | 1621-1623 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 335 |
| Issue number | 6076 |
| DOIs | |
| Publication status | Published - 30 Mar 2012 |
ASJC Scopus subject areas
- General
- History and Philosophy of Science
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Liu, F., Bakht, S., & Dean, C. (2012). Cotranscriptional role for Arabidopsis DICER-LIKE 4 in transcription termination. Science, 335(6076), 1621-1623. https://doi.org/10.1126/science.1214402