Cryptic species diversity in a widespread bumble bee complex revealed using mitochondrial DNA RFLPs

T.E. Murray, U. Fitzpatrick, M.J.F. Brown, Robert Paxton

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Cryptic species diversity is thought to be common within the class Insecta, posing problems for basic ecological and population genetic studies and conservation management. Within the temperate bumble bee (Bombus spp.) fauna, members of the subgenus Bombus sensu stricto are amongst the most abundant and widespread. However, their species diversity is controversial due to the extreme difficulty or inability morphologically to identify the majority of individuals to species. Our character-based phylogenetic analyses of partial CO1 (700 bp) from 39 individuals spread across their sympatric European ranges provided unequivocal support for five taxa (3-22 diagnostic DNA base pair sites per species). Inclusion of 20 Irish specimens to the dataset revealed >= 2.3% sequence divergence between taxa and 200 m) whilst B. cryptarum was relatively more abundant at higher altitudes. Bombus magnus was rarely encountered at urban sites. Both B. lucorum and B. terrestris are nowadays reared commercially for pollination and transported globally. Our RFLP approach to identify native fauna can underpin ecological studies of these important cryptic species as well as the impact of commercial bumble bees on them.
Original languageEnglish
Pages (from-to)653-666
Number of pages14
JournalConservation Genetics
Volume9
Issue number3
DOIs
Publication statusPublished - Jun 2008

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Nature and Landscape Conservation
  • Genetics(clinical)

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