CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin

Les J. Burke, Ru Zhang, Marek Bartkuhn, Vijay K. Tiwari, Gholamreza Tavoosidana, Sreenivasulu Kurukuti, Christine Weth, Joerg Leers, Niels Galjart, Rolf Ohlsson, Rainer Renkawitz

Research output: Chapter in Book/Report/Conference proceedingChapter

90 Citations (Scopus)

Abstract

Most of the transcription factors, RNA polymerases and enhancer binding factors are absent from condensed mitotic chromosomes. In contrast, epigenetic marks of active and inactive genes somehow survive mitosis, since the activity status from one cell generation to the next is maintained. For the zinc-finger protein CTCF, a role in interpreting and propagating epigenetic states and in separating expression domains has been documented. To test whether such a domain structure is preserved during mitosis, we examined whether CTCF is bound to mitotic chromatin. Here we show that in contrast to other zinc-finger proteins, CTCF indeed is bound to mitotic chromosomes. Mitotic binding is mediated by a portion of the zinc-finger DNA binding domain and involves sequence specific binding to target sites. Furthermore, the chromatin loop organized by the CTCF-bound, differentially methylated region at the Igf2/H19 locus can be detected in mitosis. In contrast, the enhancer/promoter loop of the same locus is lost in mitosis. This may provide a novel form of epigenetic memory during cell division.
Original languageEnglish
Title of host publicationEMBO Journal
Pages3291-3300
Number of pages10
DOIs
Publication statusPublished - 21 Sep 2005

Publication series

NameEMBO Journal
Volume24

Fingerprint

Mitosis
Chromatin
Zinc Fingers
Epigenomics
Chromosomes
DNA-Directed RNA Polymerases
Cell Division
Transcription Factors
DNA
Genes
CCCTC-binding factor

Keywords

  • CTCF
  • Cell cycle
  • Chromosome conformation capture
  • Mitotic chromosome

Cite this

Burke, L. J., Zhang, R., Bartkuhn, M., Tiwari, V. K., Tavoosidana, G., Kurukuti, S., ... Renkawitz, R. (2005). CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin. In EMBO Journal (pp. 3291-3300). (EMBO Journal; Vol. 24). https://doi.org/10.1038/sj.emboj.7600793
Burke, Les J. ; Zhang, Ru ; Bartkuhn, Marek ; Tiwari, Vijay K. ; Tavoosidana, Gholamreza ; Kurukuti, Sreenivasulu ; Weth, Christine ; Leers, Joerg ; Galjart, Niels ; Ohlsson, Rolf ; Renkawitz, Rainer. / CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin. EMBO Journal. 2005. pp. 3291-3300 (EMBO Journal).
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Burke, LJ, Zhang, R, Bartkuhn, M, Tiwari, VK, Tavoosidana, G, Kurukuti, S, Weth, C, Leers, J, Galjart, N, Ohlsson, R & Renkawitz, R 2005, CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin. in EMBO Journal. EMBO Journal, vol. 24, pp. 3291-3300. https://doi.org/10.1038/sj.emboj.7600793

CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin. / Burke, Les J.; Zhang, Ru; Bartkuhn, Marek; Tiwari, Vijay K.; Tavoosidana, Gholamreza; Kurukuti, Sreenivasulu; Weth, Christine; Leers, Joerg; Galjart, Niels; Ohlsson, Rolf; Renkawitz, Rainer.

EMBO Journal. 2005. p. 3291-3300 (EMBO Journal; Vol. 24).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin

AU - Burke, Les J.

AU - Zhang, Ru

AU - Bartkuhn, Marek

AU - Tiwari, Vijay K.

AU - Tavoosidana, Gholamreza

AU - Kurukuti, Sreenivasulu

AU - Weth, Christine

AU - Leers, Joerg

AU - Galjart, Niels

AU - Ohlsson, Rolf

AU - Renkawitz, Rainer

PY - 2005/9/21

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N2 - Most of the transcription factors, RNA polymerases and enhancer binding factors are absent from condensed mitotic chromosomes. In contrast, epigenetic marks of active and inactive genes somehow survive mitosis, since the activity status from one cell generation to the next is maintained. For the zinc-finger protein CTCF, a role in interpreting and propagating epigenetic states and in separating expression domains has been documented. To test whether such a domain structure is preserved during mitosis, we examined whether CTCF is bound to mitotic chromatin. Here we show that in contrast to other zinc-finger proteins, CTCF indeed is bound to mitotic chromosomes. Mitotic binding is mediated by a portion of the zinc-finger DNA binding domain and involves sequence specific binding to target sites. Furthermore, the chromatin loop organized by the CTCF-bound, differentially methylated region at the Igf2/H19 locus can be detected in mitosis. In contrast, the enhancer/promoter loop of the same locus is lost in mitosis. This may provide a novel form of epigenetic memory during cell division.

AB - Most of the transcription factors, RNA polymerases and enhancer binding factors are absent from condensed mitotic chromosomes. In contrast, epigenetic marks of active and inactive genes somehow survive mitosis, since the activity status from one cell generation to the next is maintained. For the zinc-finger protein CTCF, a role in interpreting and propagating epigenetic states and in separating expression domains has been documented. To test whether such a domain structure is preserved during mitosis, we examined whether CTCF is bound to mitotic chromatin. Here we show that in contrast to other zinc-finger proteins, CTCF indeed is bound to mitotic chromosomes. Mitotic binding is mediated by a portion of the zinc-finger DNA binding domain and involves sequence specific binding to target sites. Furthermore, the chromatin loop organized by the CTCF-bound, differentially methylated region at the Igf2/H19 locus can be detected in mitosis. In contrast, the enhancer/promoter loop of the same locus is lost in mitosis. This may provide a novel form of epigenetic memory during cell division.

KW - CTCF

KW - Cell cycle

KW - Chromosome conformation capture

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M3 - Chapter

C2 - 16107875

SN - 0261-4189

T3 - EMBO Journal

SP - 3291

EP - 3300

BT - EMBO Journal

ER -

Burke LJ, Zhang R, Bartkuhn M, Tiwari VK, Tavoosidana G, Kurukuti S et al. CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin. In EMBO Journal. 2005. p. 3291-3300. (EMBO Journal). https://doi.org/10.1038/sj.emboj.7600793