Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets

Leanne S Stevenson, Ken McCullough, Isabelle Vincent, Deirdre F Gilpin, Artur Summerfield, Jens Nielsen, Francis McNeilly, Brian M Adair, Gordon M Allan

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The purpose of this study was to determine serum profiles of cytokines at a protein level and Creactive protein (CRP) during the development of postweaning multisystemic wasting syndrome (PMWS) in experimentally inoculated pigs. Levels of serum IFN-alpha, IL-6, IL-10, and CRP were examined for a 35-day period in 10 piglets experimentally infected with PCV2 at 3 weeks of age. Four of the infected piglets developed severe PMWS at 14 to 21 days post-infection (d.p.i.) and died prior to termination of the experiment. The remaining six PCV2-infected piglets experienced transient fever, but did not display overt clinical signs of PMWS and were considered as subclinically infected. A bioassay was used to detect IL-6 and ELISAs were used to detect IFN-alpha, IL-10, and CRP. There were no significant differences in cytokine or CRP expression from 0 to 7 d.p.i. between the PMWS-affected and the subclinically infected piglets. Levels of IL-10 and CRP were elevated from 10 and 14 d.p.i. respectively in the PMWS-affected piglets compared to the subclinically infected piglets. There were no significant differences in IFN-alpha and IL-6 expression between the PMWS-affected piglets and the subclinically infected piglets. The present study shows that elevated levels of serum CRP and IL-10 were associated with PCV2-infected piglets that subsequently developed severe PMWS. This may help to provide further insight into the immunoaetiogenesis of this syndrome.

Original languageEnglish
Pages (from-to)189-95
Number of pages7
JournalViral Immunology
Volume19
Issue number2
DOIs
Publication statusPublished - 2006

Keywords

  • Animals
  • Animals, Newborn
  • C-Reactive Protein
  • Circoviridae Infections
  • Circovirus
  • Cytokines
  • Swine
  • Swine Diseases
  • Wasting Syndrome
  • Weaning

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