Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α- and β-zearalenol.

Oyvind L. Busk, Caroline Frizzell, Steven Verhaegen, Silvio Uhlig, Lisa Connolly, Eric Ropstad, Morten Sorlie

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

a- and b-zearalenol (a-ZOL and b-ZOL, respectively) are metabolites of the mycotoxin zearalenone (ZEN). All three individual mycotoxins have shown to be biological active i.e. being estrogenic and able to stimulate cellular proliferation albeit at different strengths. In this work, cytosol protein expression was determined by using stable-isotope labelling by amino acids in cell culture (SILAC) upon exposure of a-ZOL and b-ZOL to the steroidogenesis cell model H295R. A total of 14 and 5 individual proteins were found to be significantly regulated by a-ZOL and b-ZOL, respectively. Interestingly, there were no common protein regulations by the metabolites or the parent mycotoxin ZEN. Furthermore, the regulated proteins were assigned to networks and groups of actions that also differed from one another suggesting that the three individual mycotoxins may have unique biological activities.
Highlights

► Mycotoxins ZEN, α-ZOL, and β-ZOL regulate different proteins. ► Pleckstrin is heavily up-regulated by α- and β-ZOL. ► α- and β-ZOL promotes cellular growth and proliferation.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalToxicon
Volume59
Issue number1
Early online date20 Oct 2011
DOIs
Publication statusPublished - Jan 2012

ASJC Scopus subject areas

  • Toxicology

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