Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model

Pamela Walsh, Susan Clarke, Matt Julius, Philip Messersmith

Research output: Contribution to conferenceAbstract

Abstract

Silica additives in bone substitute materials are topical, clinically interesting and have significant support in the Orthopaedic field. Biosilica, e.g isolated from diatoms, has many advantages over its synthetic counterparts, e.g. it is amorphous, thus will be absorbed by the body, however, issues such as purity, presence of endotoxins and cytotoxicity need to be addressed before it can be further exploited. Biosilica isolated from Cyclotella Meneghiniana was then tested in a mouse model, to test the immunological response, organ toxicity (kidney, spleen, liver) and route of metabolism/excretion of silica. Five-week-old Balb-c mice were injected subcutaneously with a single high dose (50mg/ml) of Si-frustules, Si-frustules + organic linker and vehicle only control. Animals were sacrificed at 1d and 28d. The animal studies were conducted under an ethically approved protocol at Queen’s University, Belfast. The animals showed no adverse stress during the experiment and remained healthy until sacrifice. Blood results using ICP-OES analysis suggest the frustules were metabolized between comparator groups at different rates, and clearly showed elevated levels of silicon in groups injected with frustules relative to control. The histology of organs showed no variation in morphology of mice injected frustules relative compared to the control group.
Acknowledgements: The authors would like to thank Marie Curie International Outgoing Fellowships from the EU and Beaufort Marine Biodiscovery Award as part of the Marine Biotechnology Ireland Programme for providing financial support to this project.
Original languageEnglish
Publication statusPublished - 07 Jul 2015
EventMolecular Life Diatom Conference - University of Washington, Seattle, United States
Duration: 07 Jul 201510 Jul 2015
https://www.ocean.washington.edu/story/Nano+and+Biotechnology#morning2

Conference

ConferenceMolecular Life Diatom Conference
CountryUnited States
CitySeattle
Period07/07/201510/07/2015
Internet address

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Bacillariophyceae
cytotoxicity
animal models
silica
Cyclotella meneghiniana
honors and awards
nephrotoxicity
animals
orthopedics
mice
endotoxins
silicon
funding
Ireland
purity
biotechnology
histology
spleen
excretion
immune response

Cite this

Walsh, P., Clarke, S., Julius, M., & Messersmith, P. (2015). Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model. Abstract from Molecular Life Diatom Conference, Seattle, United States.
Walsh, Pamela ; Clarke, Susan ; Julius, Matt ; Messersmith, Philip. / Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model. Abstract from Molecular Life Diatom Conference, Seattle, United States.
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Walsh, P, Clarke, S, Julius, M & Messersmith, P 2015, 'Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model', Molecular Life Diatom Conference, Seattle, United States, 07/07/2015 - 10/07/2015.

Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model. / Walsh, Pamela; Clarke, Susan; Julius, Matt ; Messersmith, Philip.

2015. Abstract from Molecular Life Diatom Conference, Seattle, United States.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model

AU - Walsh, Pamela

AU - Clarke, Susan

AU - Julius, Matt

AU - Messersmith, Philip

PY - 2015/7/7

Y1 - 2015/7/7

N2 - Silica additives in bone substitute materials are topical, clinically interesting and have significant support in the Orthopaedic field. Biosilica, e.g isolated from diatoms, has many advantages over its synthetic counterparts, e.g. it is amorphous, thus will be absorbed by the body, however, issues such as purity, presence of endotoxins and cytotoxicity need to be addressed before it can be further exploited. Biosilica isolated from Cyclotella Meneghiniana was then tested in a mouse model, to test the immunological response, organ toxicity (kidney, spleen, liver) and route of metabolism/excretion of silica. Five-week-old Balb-c mice were injected subcutaneously with a single high dose (50mg/ml) of Si-frustules, Si-frustules + organic linker and vehicle only control. Animals were sacrificed at 1d and 28d. The animal studies were conducted under an ethically approved protocol at Queen’s University, Belfast. The animals showed no adverse stress during the experiment and remained healthy until sacrifice. Blood results using ICP-OES analysis suggest the frustules were metabolized between comparator groups at different rates, and clearly showed elevated levels of silicon in groups injected with frustules relative to control. The histology of organs showed no variation in morphology of mice injected frustules relative compared to the control group.Acknowledgements: The authors would like to thank Marie Curie International Outgoing Fellowships from the EU and Beaufort Marine Biodiscovery Award as part of the Marine Biotechnology Ireland Programme for providing financial support to this project.

AB - Silica additives in bone substitute materials are topical, clinically interesting and have significant support in the Orthopaedic field. Biosilica, e.g isolated from diatoms, has many advantages over its synthetic counterparts, e.g. it is amorphous, thus will be absorbed by the body, however, issues such as purity, presence of endotoxins and cytotoxicity need to be addressed before it can be further exploited. Biosilica isolated from Cyclotella Meneghiniana was then tested in a mouse model, to test the immunological response, organ toxicity (kidney, spleen, liver) and route of metabolism/excretion of silica. Five-week-old Balb-c mice were injected subcutaneously with a single high dose (50mg/ml) of Si-frustules, Si-frustules + organic linker and vehicle only control. Animals were sacrificed at 1d and 28d. The animal studies were conducted under an ethically approved protocol at Queen’s University, Belfast. The animals showed no adverse stress during the experiment and remained healthy until sacrifice. Blood results using ICP-OES analysis suggest the frustules were metabolized between comparator groups at different rates, and clearly showed elevated levels of silicon in groups injected with frustules relative to control. The histology of organs showed no variation in morphology of mice injected frustules relative compared to the control group.Acknowledgements: The authors would like to thank Marie Curie International Outgoing Fellowships from the EU and Beaufort Marine Biodiscovery Award as part of the Marine Biotechnology Ireland Programme for providing financial support to this project.

M3 - Abstract

ER -

Walsh P, Clarke S, Julius M, Messersmith P. Cytotoxicity Evaluation of Si-Diatom Frustules in a Mouse Model. 2015. Abstract from Molecular Life Diatom Conference, Seattle, United States.