Deciduous DPSCs Ameliorate MPTP-Mediated Neurotoxicity, Sensorimotor Coordination and Olfactory Function in Parkinsonian Mice

Christopher Simon, Quan Fu Gan, Premasangery Kathivaloo, Nur Afiqah Mohamad, Jagadeesh Dhamodharan, Arulmoli Krishnan, Bharathi Sengodan, Vasanth Raj Palanimuthu, Kasi Marimuthu, Heera Rajandas, Manickam Ravichandran, Sivachandran Parimannan

Research output: Contribution to journalArticle

3 Citations (Scopus)
138 Downloads (Pure)

Abstract

Parkinson's disease (PD) is a neurodegenerative disorder defined by progressive deterioration of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Dental pulp stem cells (DPSCs) have been proposed to replace the degenerated dopaminergic neurons due to its inherent neurogenic and regenerative potential. However, the effective delivery and homing of DPSCs within the lesioned brain has been one of the many obstacles faced in cell-based therapy of neurodegenerative disorders. We hypothesized that DPSCs, delivered intranasally, could circumvent these challenges. In the present study, we investigated the therapeutic efficacy of intranasally administered DPSCs in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. Human deciduous DPSCs were cultured, pre-labelled with PKH 26, and intranasally delivered into PD mice following MPTP treatment. Behavioural analyses were performed to measure olfactory function and sensorimotor coordination, while tyrosine hydroxylase (TH) immunofluorescence was used to evaluate MPTP neurotoxicity in SNpc neurons. Upon intranasal delivery, degenerated TH-positive neurons were ameliorated, while deterioration in behavioural performances was significantly enhanced. Thus, the intranasal approach enriched cell delivery to the brain, optimizing its therapeutic potential through its efficacious delivery and protection against dopaminergic neuron degeneration.

Original languageEnglish
Article number568
Number of pages13
JournalInternational journal of molecular sciences
Volume20
Issue number3
DOIs
Publication statusPublished - 29 Jan 2019

Keywords

  • behavioural analysis
  • dental pulp stem cells
  • intranasal delivery
  • MPTP
  • Parkinson’s disease
  • tyrosine hydroxylase

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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