Deciphering the Gene Regulatory Landscape Encoded in DNA Biophysical Features

Abhijeet Pataskar, Willem Vanderlinden, Johannes Emmerig, Aditi Singh, Jan Lipfert, Vijay K. Tiwari*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
191 Downloads (Pure)


Gene regulation in higher organisms involves a sophisticated interplay between genetic and epigenetic mechanisms. Despite advances, the logic in selective usage of certain genomic regions as regulatory elements remains unclear. Here we show that the inherent biophysical properties of the DNA encode epigenetic state and the underlying regulatory potential. We find that the propeller twist (ProT) level is indicative of genomic location of the regulatory elements, their strength, the affinity landscape of transcription factors, and distribution in the nuclear 3D space. We experimentally show that ProT levels confer increased DNA flexibility and surface accessibility, and thus potentially primes usage of high ProT regions as regulatory elements. ProT levels also correlate with occurrence and phenotypic consequences of mutations. Interestingly, cell-fate switches involve a transient usage of low ProT regulatory elements. Altogether, our work provides unprecedented insights into the gene regulatory landscape encoded in the DNA biophysical features.
Original languageEnglish
Pages (from-to)638-649
Number of pages12
Early online date31 Oct 2019
Publication statusPublished - 22 Nov 2019


  • Biophysics
  • Genetics
  • Molecular Genetics

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Deciphering the Gene Regulatory Landscape Encoded in DNA Biophysical Features'. Together they form a unique fingerprint.

Cite this