Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver b-glucosidase activity

Andrea J Day; M.Susan DuPont; Saxon Ridley; Mike Rhodes; Michael J.C. Rhodes; Michael R.A. Morgan; Gary Williamson

doi:10.1016/S0014-5793(98)01101-6

Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver b-glucosidase activity

Andrea J Day, M.Susan DuPont, Saxon Ridley, Mike Rhodes, Michael J.C. Rhodes, Michael R.A. Morgan, Gary Williamson

Research output: Contribution to journal › Article › peer-review

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Abstract

Flavonoid and isoflavonoid glycosides are common dietary phenolics which may be absorbed from the small intestine of humans. The ability of cell-free extracts from human small intestine and liver to deglycosylate various (iso)flavonoid glycosides was investigated. Quercetin 4'-glucoside, naringenin 7-glucoside, apigenin 7-glucoside, genistein 7-glucoside and daidzein 7-glucoside were rapidly deglycosylated by both tissue extracts, whereas quercetin 3,4'- diglucoside, quercetin 3-glucoside, kaempferol 3-glucoside, quercetin 3- rhamnoglucoside and naringenin 7-rhamnoglucoside remained unchanged. The Km for hydrolysis of quercetin 4'-glucoside and genistein 7- glucoside was approximately 32+/-12 and approximately 14+/-3 microM in both tissues respectively. The enzymatic activity of the cell-free extracts exhibits similar properties to the cytosolic broad-specificity -glucosidase previously described in mammals.

Original language	English
Pages (from-to)	71-75
Number of pages	5
Journal	FEBS Letters
Volume	436
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(98)01101-6
Publication status	Published - 20 Oct 1998
Externally published	Yes

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@article{01793de16692413a9d50eb344341ba1f,

title = "Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver b-glucosidase activity",

abstract = "Flavonoid and isoflavonoid glycosides are common dietary phenolics which may be absorbed from the small intestine of humans. The ability of cell-free extracts from human small intestine and liver to deglycosylate various (iso)flavonoid glycosides was investigated. Quercetin 4'-glucoside, naringenin 7-glucoside, apigenin 7-glucoside, genistein 7-glucoside and daidzein 7-glucoside were rapidly deglycosylated by both tissue extracts, whereas quercetin 3,4'- diglucoside, quercetin 3-glucoside, kaempferol 3-glucoside, quercetin 3- rhamnoglucoside and naringenin 7-rhamnoglucoside remained unchanged. The Km for hydrolysis of quercetin 4'-glucoside and genistein 7- glucoside was approximately 32+/-12 and approximately 14+/-3 microM in both tissues respectively. The enzymatic activity of the cell-free extracts exhibits similar properties to the cytosolic broad-specificity -glucosidase previously described in mammals.",

author = "Day, \{Andrea J\} and M.Susan DuPont and Saxon Ridley and Mike Rhodes and Rhodes, \{Michael J.C.\} and Morgan, \{Michael R.A.\} and Gary Williamson",

year = "1998",

month = oct,

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doi = "10.1016/S0014-5793(98)01101-6",

language = "English",

volume = "436",

pages = "71--75",

journal = "FEBS Letters",

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publisher = "John Wiley \& Sons Inc.",

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TY - JOUR

T1 - Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver b-glucosidase activity

AU - Day, Andrea J

AU - DuPont, M.Susan

AU - Ridley, Saxon

AU - Rhodes, Mike

AU - Rhodes, Michael J.C.

AU - Morgan, Michael R.A.

AU - Williamson, Gary

PY - 1998/10/20

Y1 - 1998/10/20

N2 - Flavonoid and isoflavonoid glycosides are common dietary phenolics which may be absorbed from the small intestine of humans. The ability of cell-free extracts from human small intestine and liver to deglycosylate various (iso)flavonoid glycosides was investigated. Quercetin 4'-glucoside, naringenin 7-glucoside, apigenin 7-glucoside, genistein 7-glucoside and daidzein 7-glucoside were rapidly deglycosylated by both tissue extracts, whereas quercetin 3,4'- diglucoside, quercetin 3-glucoside, kaempferol 3-glucoside, quercetin 3- rhamnoglucoside and naringenin 7-rhamnoglucoside remained unchanged. The Km for hydrolysis of quercetin 4'-glucoside and genistein 7- glucoside was approximately 32+/-12 and approximately 14+/-3 microM in both tissues respectively. The enzymatic activity of the cell-free extracts exhibits similar properties to the cytosolic broad-specificity -glucosidase previously described in mammals.

AB - Flavonoid and isoflavonoid glycosides are common dietary phenolics which may be absorbed from the small intestine of humans. The ability of cell-free extracts from human small intestine and liver to deglycosylate various (iso)flavonoid glycosides was investigated. Quercetin 4'-glucoside, naringenin 7-glucoside, apigenin 7-glucoside, genistein 7-glucoside and daidzein 7-glucoside were rapidly deglycosylated by both tissue extracts, whereas quercetin 3,4'- diglucoside, quercetin 3-glucoside, kaempferol 3-glucoside, quercetin 3- rhamnoglucoside and naringenin 7-rhamnoglucoside remained unchanged. The Km for hydrolysis of quercetin 4'-glucoside and genistein 7- glucoside was approximately 32+/-12 and approximately 14+/-3 microM in both tissues respectively. The enzymatic activity of the cell-free extracts exhibits similar properties to the cytosolic broad-specificity -glucosidase previously described in mammals.

U2 - 10.1016/S0014-5793(98)01101-6

DO - 10.1016/S0014-5793(98)01101-6

M3 - Article

SN - 0014-5793

VL - 436

SP - 71

EP - 75

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver b-glucosidase activity

Abstract

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