Deposition of multilayer coatings onto highly porous materials by Layer-by-Layer assembly for bone tissue engineering applications using cyclic mechanical deformation and perfusion

Mohammad Ali Sahebalzamani, Tina Sadat Hashemi, Zohreh Mousavi Nejad, Srishti Agarwal, Helen O. McCarthy, Tanya J. Levingstone, Nicholas J. Dunne*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

By using Layer-by-Layer (LbL) assembly, micro- and nano-scale coatings can be applied to porous structures, allowing for the potential customisation of scaffolds for bone tissue engineering applications. In this study, we developed a purpose-designed LbL assembly system, enabling continuous perfusion flow and cyclic compression, to fabricate LbL multilayer-coated scaffolds with tailored properties. Their physicochemical properties were analysed using SEM and FTIR spectroscopy, while their elastic compressive modulus quantified their mechanical performance. This study compared immersion alone (i.e., static conditions) to the combination of perfusion flow (12 mL min−1, 10 rpm) and cyclic compressive loading (5% strain, 1 Hz) (i.e., dynamic conditions) as methods to influence coating deposition during LbL assembly. The results demonstrated that the LbL-coated scaffolds with 40-multilayer coatings deposited under dynamic conditions demonstrated a 40-fold improvement in the compressive elastic modulus compared to uncoated scaffolds and a 16-fold increase was achieved when the LbL coatings were applied under static conditions. Importantly, application of the dynamic coating conditions during the LbL assembly process preserved the high porosity and interconnectivity of the scaffolds even after applying the 40-multilayer coating. Moreover, the nanocomposite coatings enhanced surface characteristics such as roughness and hydrophilicity. Taken together, adoption of the proposed approach of combining perfusion flow and cyclic compression loading during assembly of LbL-coated scaffolds is a promising approach for bone tissue engineering applications.

Original languageEnglish
JournalMaterials Advances
Early online date01 Feb 2024
DOIs
Publication statusEarly online date - 01 Feb 2024

Bibliographical note

Funding Information:
The research conducted in this publication was funded by the Irish Research Council (Government of Ireland Scholarship Scheme) under award number GOIPG/2020/1344. The FTIR and contact angle analyses were carried at the Nano Research Facility (NRF) in Dublin City University (DCU), which was funded under the Programme for Research in Third Level Institutions (PRTLI) Cycle 5. The PRTLI is co-funded through the European Regional Development Fund (ERDF), part of the European Union Structural Funds Programme 2011–2015.

Publisher Copyright:
© 2024 RSC.

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • General Materials Science

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