Derivation of clinically compliant MSCs from CD105+, CD24-differentiated human ESCs

Q.Z. Lian, E. Lye, K.S. Yeo, E.K.W. Tan, Manuel Salto-Tellez, T.M. Liu, N. Palanisamy, R.M. El Oakley, E.H. Lee, B. Lim, S.K. Lim

Research output: Contribution to journalArticlepeer-review

238 Citations (Scopus)

Abstract

Adult tissue-derived mesenchymal stem cells ( MSCs) have demonstrated therapeutic efficacy in treating diseases or repairing damaged tissues through mechanisms thought to be mediated by either cell replacement or secretion of paracrine factors. Characterized, self- renewing human ESCs could potentially be an invariable source of consistently uniform MSCs for therapeutic applications. Here we describe a clinically relevant and reproducible manner of generating identical batches of hESC- derived MSC ( hESC- MSC) cultures that circumvents exposure to virus, mouse cells, or serum. Trypsinization and propagation of HuES9 or H1 hESCs in feeder- and serum-free selection media generated three polyclonal, karyotypically stable, and phenotypically MSC-like cultures that do not express pluripotency- associated markers but displayed MSC- like surface antigens and gene expression profile. They differentiate into adipocytes, osteocytes, and chondrocytes in vitro. Gene expression and fluorescence- activated cell sorter analysis identified CD105 and CD24 as highly expressed antigens on hESC- MSCs and hESCs, respectively. CD105+, CD24- monoclonal isolates have a typical MSC gene expression profiles and were identical to each other with a highly correlated gene expression profile ( r(2) >.90). We have developed a protocol to reproducibly generate clinically compliant and identical hESC- MSC cultures.
Original languageEnglish
Pages (from-to)425-436
Number of pages12
JournalStem Cells
Volume25
Issue number2
DOIs
Publication statusPublished - Feb 2007

ASJC Scopus subject areas

  • Cell Biology

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