Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.
|Number of pages||11|
|Journal||Methods in Molecular Biology|
|Early online date||03 Feb 2012|
|Publication status||Published - 2012|
ASJC Scopus subject areas
- Molecular Biology