Detection of cathepsin S cysteine protease in human brain tumour microdialysates in vivo.

T. Flannery, R. S. McConnell, S. McQuaid, G. McGregor, M. Mirakhur, L. Martin, C. Scott, R. Burden, B. Walker, C. McGoohan, P. Johnston

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Microdialysis enables the chemistry of extracellular ?uid in body tissues to be measured. Extracellular proteases such as the cysteine protease, cathepsin S (CatS), are thought to facilitate astrocytoma invasion. Microdialysates obtained from human brain tumoursin vivo were subjected to cathepsin S activity and ELISA assays. Cathepsin S ELISA expression was detected in ?ve out of 10 tumour microdialysates, while activity was detected in ?ve out of 11 tumour microdialysates. Cathepsin S expression was also detected in microdialysate from the normal brain control although no activity was found in the same sample. While some re?nements to the technique are necessary, the authors demonstrate the feasibility and safety of microdialysis in human astrocytomasin vivo. Characterisation of the extracellular environment of brain tumoursin vivo using microdialysis may be a useful tool to identify the protease pro?le of brain tumours.
Original languageEnglish
Pages (from-to)204-209
Number of pages6
JournalBritish Journal of Neurosurgery
Volume21
Issue number2
DOIs
Publication statusPublished - Apr 2007

ASJC Scopus subject areas

  • Clinical Neurology

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