Detection of epidermal growth factor receptor variations by partially denaturing HPLC

T.M. Chin, D. Anuar, R. Soo, Manuel Salto-Tellez, W.Q. Li, B. Ahmad, S.C. Lee, B.C. Goh, K. Kawakami, A. Segal, B. Iacopetta, R. Soong

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Background: Epidermal growth factor receptor gene (EGFR) variants may be useful markers for identifying responders to gefitinib and erlotinib, small-molecule tyrosine kinase inhibitors of EGFR; therefore, sensitive and cost-effective assays are needed to detect EGFR variants in routine clinical samples. We have developed a partially denaturing HPLC (pDHPLC) assay that is superior to direct sequencing with respect to detection limits, costs, and time requirements.
Original languageEnglish
Pages (from-to)62-70
Number of pages9
JournalClinical Chemistry
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 2007

ASJC Scopus subject areas

  • Clinical Biochemistry

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