Detection of ivermectin residues in bovine liver using an enzyme immunoassay

Joan Crooks, Robert Baxter, Sandra Traynor, Christopher Elliott, Derek McCaughey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Ivermectin, a member of the avermectin group, is frequently used to control parasites in many food producing animal species. A method for the detection and quantification of ivermectin residues in bovine liver has been developed. Liver samples (4 g) were extracted with acetonitrile and applied to a competitive enzyme immunoassay using a polyclonal antiserum raised in rabbits against an ivermectin-transferrin conjugate, The limit of detection of the assay (mean +/- 3s) calculated from the analysis of 24 known negative samples was 1.6 ng g(-1), Intra- and inter-assay RSDs were determined as 8.8 and 14.6%, respectively, using a negative bovine liver sample fortified with 100 ng g(-1) of ivermectin. Four Friesian steers were treated with a pour-on application of ivermectin at a dose rate of 0.5 mg kg(-1) body mass then withdrawn and killed at 7, 14, 21 and 28 d, Livers mere removed and ivermectin residue concentrations determined using the proposed immunoassay procedure, Seven days post-treatment the ivermectin liver concentration was determined as 52.7 ng g(-1), decreasing to 4.1 ng(-1) at 28 d, All immunoassay results were confirmed using high-performance liquid chromatography (HPLC), The immunoassay and HPLC results for invermectin ranged from 1 to 58 ng g(-1) and were in close correlation (r = 0.99).
Original languageEnglish
Pages (from-to)355-358
Number of pages4
JournalAnalyst
Volume123
Issue number2
Publication statusPublished - Feb 1998

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Detection of ivermectin residues in bovine liver using an enzyme immunoassay'. Together they form a unique fingerprint.

  • Cite this

    Crooks, J., Baxter, R., Traynor, S., Elliott, C., & McCaughey, D. (1998). Detection of ivermectin residues in bovine liver using an enzyme immunoassay. Analyst, 123(2), 355-358.