Detection of pathogen based on the catalytic growth of gold nanocrystals

Xin Xing Li, Cuong Cao, Se Jong Han, Sang Jun Sim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A homogenous detection of pathogen (Giardia lamblia cysts) based on the catalytic growth of gold nanoparticles (AuNPs) has been studied. In this study, centrifugal filters were employed as tools to concentrate and separate the pathogen cells, and moreover amplify the detection signal. The catalytic growth of gold nanoparticles was verified to be positively related to gold seeds concentration. On this basis, homogenous detection of the pathogenic bacteria in liquid phase was established by means of conjugating antibody to gold seeds. Under the given experimental condition, detection limit of G. lamblia cysts was determined as low as 1.088 × 103 cells ml-1. The additional nonspecific binding tests were also conducted to verify the detection specificity. This sensing platform has been proved to be a sensitive, reliable and simple method for large-scale pathogen detection, and provide valuable insight for the development of gold nanocrystals based colorimetric biosensors.
Original languageEnglish
Pages (from-to)1425-1431
Number of pages7
JournalWater Research
Volume43
Issue number5
Publication statusPublished - 2009

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