Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool

Sandra Leonardo, Sotiris Kiparissis, Maria Rambla-Alegre, Sergi Almarza, Ana Roque, Karl B. Andree, Aris Christidis, Cintia Flores, Josep Caixach, Katrina Campbell, Christopher T. Elliott, Katerina Aligizaki, Jorge Diogène, Mònica Campàs

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6 Citations (Scopus)
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Abstract

Two small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs), potent neurotoxins that constitute a food safety hazard when present in seafood. This tool revealed the presence of TTXs in both individuals. Results were compared with those provided by mELISA and LC-HRMS, the latter confirming the presence of TTX. Some of the tissues contained TTX contents close to or above 2 mg/kg. L. sceleratus juveniles had been considered as non-toxic and, to our knowledge, this is the first report of high TTX levels in small L. sceleratus individuals. Such specimens can be mistaken with other edible species, posing a threat to consumers. The availability of low-cost and user-friendly tools for TTXs detection will contribute to guarantee seafood safety.
Original languageEnglish
Pages (from-to)255 - 262
JournalFood Chemistry
Volume290
Early online date29 Mar 2019
DOIs
Publication statusPublished - 30 Aug 2019

Keywords

  • Tetrodotoxin, Electrochemical, Immunoassay, Magnetic bead, Liquid chromatography-high resolution mass spectrometry (LC-HRMS), Pufferfish, Juvenile,

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