Determination of 42 mycotoxins in oats using a mechanically assisted QuEChERS sample preparation and UHPLC-MS/MS detection

Lorenzo De Colli*, Christopher Elliott, John Finnan, Jim Grant, Elke K. Arendt, Susan P. McCormick, Martin Danaher

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A method was developed and validated for the simultaneous determination of 42 mycotoxins in oats. The method includes all the mycotoxins listed under Commission Regulation 1881/2006 and Commission Recommendation 165/2013, the emerging mycotoxins (beauvericin, alternariol, alternariol-methyl-ether and enniatins), and two masked metabolites, namely deoxynivalenol-3-glucoside and T-2-glucoside. The method also focuses on a wide range of analytes of toxicological interest. The sample preparation involved extraction with an aqueous acetic acid solution and acetonitrile, followed by QuEChERS with mechanically assisted vibrational shaking. No further clean-up steps were employed, and analysis was performed using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Trueness ranged between 78% and 158%, while precision ranged from 1.7% to 49.9% under within-laboratory reproducibility conditions. Beside the high degree of accuracy and sample throughput provided, the method can be applied to a large number of compounds currently not regulated, thus generating knowledge and for risk assessment purposes.

Original languageEnglish
Article number122187
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1150
Early online date22 May 2020
DOIs
Publication statusPublished - 01 Aug 2020

Keywords

  • Emerging
  • Masked
  • Mechanically-assisted QuEChERS
  • Mycotoxins
  • Oats
  • UHPLC-MS/MS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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