Determination of cytokine regulated glycan expression by using molecularly imprinted polymers targeting sialic acid

Sudhirkumar Shinde, Zahra El-Schich

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Abstract

Cancer cells often have an increased amount of glycans, such as sialic acid (SA), on the cell surface, which normally
play an important role in cell growth, proliferation and differentiation. In this study, SA expression is determined
by fluorescent nanoprobes, molecularly imprinted polymers, SA-MIPs. The nanoprobes are synthesized with an
imprinting approach to produce tailor-made fluorescent core-shell particles with high affinity for cell surface SA.
Inflammation and cytokine production are well known tumor promoters, modulating the cellular microenvironment,
including an aberrant cell surface glycan pattern. The recombinant cytokines IL-4, IL-6, IL-8 and a cocktail of
cytokines collected from stimulated T leukemia Jurkat cells were used to induce in vitro inflammation in two cell
lines, and thereafter analyzed with the use of SA-MIPs and flow cytometry. One of the cell lines showed a different
binding pattern of SA-MIPs after treatment with recombinant cytokines and the cytokine cocktail. This study shows
that SA-MIPs can be an important tool in the investigation of overexpressed glycans in the tumor microenvironment.
Original languageEnglish
Article number56
Number of pages9
JournalJournal of Cancer Metastasis and Treatment
Volume5
Publication statusPublished - 11 Jul 2019

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