TY - JOUR
T1 - Determination of deoxynivalenol in cereals and cereal products by immunoaffinity column cleanup with liquid chromatography
T2 - Interlaboratory study
AU - MacDonald, Susan J.
AU - Chan, Danny
AU - Brereton, Paul
AU - Damant, Andrew
AU - Wood, Roger
AU - Dao Duy, K.
AU - Felgueiras, I.
AU - Feron, T.
AU - Herry, M. P.
AU - Ioannou-Kakouri, E.
AU - Koch, P.
AU - Krska, R.
AU - Majerus, P.
AU - Nesbit, J.
AU - Reuter, M.
AU - Stangroom, S.
AU - Symonds, P.
AU - Weber, R.
PY - 2005/7/1
Y1 - 2005/7/1
N2 - An interlaboratory study was performed on behalf of the UK Food Standards Agency to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatographic (LC) method for the determination of deoxynivalenol in a variety of cereals and cereal products at proposed European regulatory limits. The test portion was extracted with water. The sample extract was filtered and applied to an immunoaffinity column. After being washed with water, the deoxynivalenol was eluted with acetonitrile or methanol. Deoxynivalenol was quantitated by reversed-phase LC with UV determination. Samples of artificially contaminated wheat-flour, rice flour, oat flour, polenta, and a wheat based breakfast cereal, naturally contaminated wheat flour, and blank (very low level) samples of each matrix were sent to 13 collaborators in 7 European countries. Participants were asked to spike test portions of all samples at a range of deoxynivalenol concentrations equivalent to 200-2000 ng/g deoxynivalenol. Average recoveries ranged from 78 to 87%. Based on results for 6 artificially contaminated samples (blind duplicates), the relative standard deviation for repeatability (RSD r) ranged from 3.1 to 14.1%, and the relative standard deviation for reproducibility (RSDR) ranged from 11.5 to 26.3%. The method showed acceptable within-laboratory and between-laboratory precision for all 5 matrixes, as evidenced by HorRat values <1.3.
AB - An interlaboratory study was performed on behalf of the UK Food Standards Agency to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatographic (LC) method for the determination of deoxynivalenol in a variety of cereals and cereal products at proposed European regulatory limits. The test portion was extracted with water. The sample extract was filtered and applied to an immunoaffinity column. After being washed with water, the deoxynivalenol was eluted with acetonitrile or methanol. Deoxynivalenol was quantitated by reversed-phase LC with UV determination. Samples of artificially contaminated wheat-flour, rice flour, oat flour, polenta, and a wheat based breakfast cereal, naturally contaminated wheat flour, and blank (very low level) samples of each matrix were sent to 13 collaborators in 7 European countries. Participants were asked to spike test portions of all samples at a range of deoxynivalenol concentrations equivalent to 200-2000 ng/g deoxynivalenol. Average recoveries ranged from 78 to 87%. Based on results for 6 artificially contaminated samples (blind duplicates), the relative standard deviation for repeatability (RSD r) ranged from 3.1 to 14.1%, and the relative standard deviation for reproducibility (RSDR) ranged from 11.5 to 26.3%. The method showed acceptable within-laboratory and between-laboratory precision for all 5 matrixes, as evidenced by HorRat values <1.3.
UR - http://www.scopus.com/inward/record.url?scp=25444464392&partnerID=8YFLogxK
M3 - Article
C2 - 16152940
AN - SCOPUS:25444464392
SN - 1060-3271
VL - 88
SP - 1197
EP - 1204
JO - Journal of AOAC International
JF - Journal of AOAC International
IS - 4
ER -