Determination of deoxynivalenol in cereals and cereal products by immunoaffinity column cleanup with liquid chromatography: Interlaboratory study

Susan J. MacDonald*, Danny Chan, Paul Brereton, Andrew Damant, Roger Wood, K. Dao Duy, I. Felgueiras, T. Feron, M. P. Herry, E. Ioannou-Kakouri, P. Koch, R. Krska, P. Majerus, J. Nesbit, M. Reuter, S. Stangroom, P. Symonds, R. Weber

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

An interlaboratory study was performed on behalf of the UK Food Standards Agency to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatographic (LC) method for the determination of deoxynivalenol in a variety of cereals and cereal products at proposed European regulatory limits. The test portion was extracted with water. The sample extract was filtered and applied to an immunoaffinity column. After being washed with water, the deoxynivalenol was eluted with acetonitrile or methanol. Deoxynivalenol was quantitated by reversed-phase LC with UV determination. Samples of artificially contaminated wheat-flour, rice flour, oat flour, polenta, and a wheat based breakfast cereal, naturally contaminated wheat flour, and blank (very low level) samples of each matrix were sent to 13 collaborators in 7 European countries. Participants were asked to spike test portions of all samples at a range of deoxynivalenol concentrations equivalent to 200-2000 ng/g deoxynivalenol. Average recoveries ranged from 78 to 87%. Based on results for 6 artificially contaminated samples (blind duplicates), the relative standard deviation for repeatability (RSD r) ranged from 3.1 to 14.1%, and the relative standard deviation for reproducibility (RSDR) ranged from 11.5 to 26.3%. The method showed acceptable within-laboratory and between-laboratory precision for all 5 matrixes, as evidenced by HorRat values <1.3.

Original languageEnglish
Pages (from-to)1197-1204
Number of pages8
JournalJournal of AOAC International
Volume88
Issue number4
Publication statusPublished - 01 Jul 2005

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

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