A chemical-derivatization-triggered aggregation-induced emission (AIE) method for the highly selective determination of hydrogen sulfide (H2S) in wine matrices by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed. The detection strategy was developed based on the chemical derivatization of H2S using a low-cost AIE-active fluorescence derivatization reagent, N-(3-iodine-2-oxopropyl)pyrene methamine (NIPM), to trigger specific AIE at 475 nm, which was red-shifted sharply to the maximum emission wavelength as compared with NIPM monomers of 375 nm, effectively quenching the interference from other thiol-containing compounds. With the aid of specific AIE and the effective separation of HPLC, the proposed method showed high selectivity and sensitivity toward H2S. The limits of detection (LODs) at the sub-nM level of 0.25 nmol/L in the wine-beer sample and 0.30 nmol/L in red wine sample were obtained. To certify its applicability, this proposed strategy was successfully applied for the determination of H2S in wine matrices.
|Number of pages||8|
|Journal||Journal of agricultural and food chemistry|
|Early online date||31 Oct 2019|
|Publication status||Published - 22 Jan 2020|
Bibliographical noteFunding Information:
This research was funded by the National Natural Science Foundation of China (21677085 and 31801454), the Natural Science Foundation of Shandong Province (ZR2017JL012), the Science and Technology Nova Plan of Shaanxi Province (2019KJXX-010), and the Youth Innovation Team of Shaanxi Universities (Food Quality and Safety Innovation Team).
Copyright © 2019 American Chemical Society.
- aggregation-induced emission
- fluorescence detection
- high-performance liquid chromatography
- Hydrogen sulfide
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)