Determination of metformin in plasma using a new ion pair solid phase extraction technique and ion pair liquid chromatography

S. AbuRuz, Jeffrey Millership, James McElnay

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

This article describes the development of the first ion pair solid phase extraction technique (IPSPE), which has been applied to the extraction of metformin from plasma samples. In addition an ion pair chromatographic method was developed for the specific HPLC determination of metformin. Several extraction and HPLC methods have been described previously for metformin, however, most of them did not solve the problems associated with the high polarity of this drug. Drug recovery in the developed method was found to be more than 98%. The limit of detection and limit of quantification was 3 and 5 ng/ml, respectively. The intraday and interday precision (measured by coefficient of variation, CV%) was always less than 9%. The accuracy (measured by relative error, R.E.%) was always less than 6.9%. Stability analysis showed that metformin is stable for at least 3 months when stored at -70degreesC. The method has been applied to 150 patient samples as part of a medication adherence study. (C) 2003 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume798
Issue number2
DOIs
Publication statusPublished - 25 Dec 2003

ASJC Scopus subject areas

  • Biochemistry

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