Determination of pantothenic acid in foods by optical biosensor immunoassay

Simon A Haughey, Anthony A O'Kane, G Andrew Baxter, Andras Kalman, Marie-José Trisconi, Harvey E Indyk, Gaylene A Watene

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23 Citations (Scopus)

Abstract

An optical biosensor inhibition immunoassay was developed using a specific pantothenic acid-binding protein for the quantitation of free pantothenic acid (vitamin B5) in foodstuffs. Samples were prepared by a simple extraction procedure in buffer, and vitamin content was estimated against authentic calibrants in the same buffer. Performance parameters included a working range of 10-5000 ng/mL, a limit of detection of 4.4 ng/mL, precision relative standard deviation of 5.4-7.1% over a range of concentrations, and recoveries > 95% in the matrixes tested. A wide range of foodstuffs, including National Institute of Standards and Technology reference samples, were tested in 3 independent laboratories and the results were compared with microbiological assay and liquid chromatography/mass spectrometry (LC/MS) methods. The results indicate that the biosensor technique is appropriate for the estimation of pantothenic acid in a wide range of foodstuffs.

Original languageEnglish
Pages (from-to)1008-14
Number of pages7
JournalJournal of AOAC International
Volume88
Issue number4
Publication statusPublished - 13 Sep 2005

Keywords

  • Biosensing Techniques
  • Calibration
  • Chromatography, Liquid
  • Dietary Supplements
  • Dose-Response Relationship, Drug
  • Food Analysis
  • Hydrogen-Ion Concentration
  • Immunoassay
  • Infant Formula
  • Mass Spectrometry
  • Pantothenic Acid
  • Surface Plasmon Resonance
  • Journal Article

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  • Cite this

    Haughey, S. A., O'Kane, A. A., Baxter, G. A., Kalman, A., Trisconi, M-J., Indyk, H. E., & Watene, G. A. (2005). Determination of pantothenic acid in foods by optical biosensor immunoassay. Journal of AOAC International, 88(4), 1008-14.