Determination of Survival of Wildtype and Mutant Escherichia coli in Soil

Yinka Somorin, Conor O'Byrne

Research output: Contribution to journalArticle

Abstract

E. coli resides in the gastrointestinal tract of humans and other warm-blooded animals but recent studies have shown that E. coli can persist and grow in various external environments including soil. The general stress response regulator, RpoS, helps E. coli overcome various stresses, however its role in soil survival was unknown. This soil survival assay protocol was developed and used to determine the role of the general stress response regulator, RpoS, in the survival of E. coli in soil. Using this soil survival assay, we demonstrated that RpoS was important for the survival of E. coli in soil. This protocol describes the development of the soil survival assay especially the recovery of E. coli inoculated into soil and can be adapted to allow further investigations into the survival of other bacteria in soil.
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBio-protocol
Volume7
Issue number14
DOIs
Publication statusPublished - 20 Jul 2017

Keywords

  • 1996
  • 2003
  • background
  • bacteria recovery
  • belonging to the
  • berg
  • enterobacteriaceae family
  • environmental persistence
  • escherichia coli
  • escherichia coli is a
  • facultative anaerobe
  • gordon and cowling
  • gram-negative
  • it can be transferred
  • reptiles
  • rpos
  • soil survival
  • through water and sediments
  • tract of humans
  • via
  • warm-blooded animals and
  • which inhabits the intestinal

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