Determination of Zn, Cu and Fe in human patients' serum using micro-sampling ICP-MS and sample dilution

Tharwat N. Abduljabbar; Barry L. Sharp; Helen J. Reid; Neda Barzegar-Befroeid; Tunde Peto; Imre Lengyel

doi:10.1016/j.talanta.2019.05.098

Determination of Zn, Cu and Fe in human patients' serum using micro-sampling ICP-MS and sample dilution

Tharwat N. Abduljabbar^*, Barry L. Sharp, Helen J. Reid, Neda Barzegar-Befroeid, Tunde Peto, Imre Lengyel

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 μL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO₃. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.

Original language	English
Pages (from-to)	663-669
Number of pages	7
Journal	Talanta
Volume	204
Early online date	31 May 2019
DOIs	https://doi.org/10.1016/j.talanta.2019.05.098
Publication status	Published - 01 Nov 2019

Keywords

AD
AMD
ICP-MS
Serum analysis
Trace elements

ASJC Scopus subject areas

Analytical Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Determination of Zn, Cu and Fe in human patients' serum using micro-sampling ICP-MS and sample dilution",

abstract = "A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 μL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO3. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.",

keywords = "AD, AMD, ICP-MS, Serum analysis, Trace elements",

author = "Abduljabbar, {Tharwat N.} and Sharp, {Barry L.} and Reid, {Helen J.} and Neda Barzegar-Befroeid and Tunde Peto and Imre Lengyel",

year = "2019",

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doi = "10.1016/j.talanta.2019.05.098",

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T1 - Determination of Zn, Cu and Fe in human patients' serum using micro-sampling ICP-MS and sample dilution

AU - Abduljabbar, Tharwat N.

AU - Sharp, Barry L.

AU - Reid, Helen J.

AU - Barzegar-Befroeid, Neda

AU - Peto, Tunde

AU - Lengyel, Imre

PY - 2019/11/1

Y1 - 2019/11/1

N2 - A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 μL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO3. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.

AB - A high-throughput, sensitive and rapid method was developed for the determination of Zn, Cu and Fe in small volumes (30 μL) of human serum using inductively coupled plasma mass spectrometry (ICP-MS). The sample preparation procedure employed simple 100-fold dilution of the serum samples with 1.0% butanol, 0.5% v/v ammonia, 0.02% v/v Triton X-100 and 0.01% v/v HNO3. The reliability of the method was evaluated using serum UTAK certified reference material, and the results matched well with the certified values. The method was applied to determine Zn, Cu and Fe in 81 human serum samples from participants in Alzheimer disease (AD) and age-related macular degeneration (AMD) studies. No significant differences were found in Zn and Cu levels between age matched controls, AD and AMD patients. Whilst iron levels appeared marginally higher in the AMD group, compared with the AD group, iron showed larger overall variability than the other two elements.

KW - AD

KW - AMD

KW - ICP-MS

KW - Serum analysis

KW - Trace elements

U2 - 10.1016/j.talanta.2019.05.098

DO - 10.1016/j.talanta.2019.05.098

M3 - Article

AN - SCOPUS:85067439457

SN - 0039-9140

VL - 204

SP - 663

EP - 669

JO - Talanta

JF - Talanta

ER -

Determination of Zn, Cu and Fe in human patients' serum using micro-sampling ICP-MS and sample dilution

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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