Development and validation of a dried blood spot LC-MS/MS assay to quantify ranitidine in paediatric samples.

Shirish Yakkundi, Jeffrey Millership, Paul Collier, Michael Shields, James McElnay

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A novel approach has been developed to determine ranitidine in paediatric samples using dried blood spots (DBS) on Guthrie cards (Whatman 903). A selective and sensitive HPLC-MS/MS assay has been developed and validated using small volumes of blood (30µl). A 6mm disc was punched from each DBS and extracted with methanolic solution of the internal standard (IS) nizatidine. This was further subjected to solid phase extraction (SPE), followed by reversed phase HPLC separation, using a XBridge™ C18 column and mobile phase 10mM ammonium acetate/methanol (98:2 v/v) with a flow rate of 0.3mL/min. This was combined with multiple reaction monitoring (MRM) mass detection using electrospray ionisation (ESI). The calibration curve for ranitidine was found linear over the range 10-500ng/mL (r=0.996). The limit of quantification (LOQ) of the method was validated at 10ng/mL. Accuracy and precision values for within and between days were
Original languageEnglish
Pages (from-to)1057-1063
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume56(5)
Issue number5
DOIs
Publication statusPublished - 15 Aug 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Development and validation of a dried blood spot LC-MS/MS assay to quantify ranitidine in paediatric samples.'. Together they form a unique fingerprint.

  • Cite this