Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species

Silvina P. Brengi, Stephen B. O'Brien, Mariana Pichel, Carol Iversen, Matthew Arduino, Norma Binsztein, Bette Jensen, Franco Pagotto, Efrain M. Ribot, Roger Stephan, Nicole Cernela, Kara Cooper, Seamus Fanning*

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalFoodborne pathogens and disease
Volume9
Issue number9
DOIs
Publication statusPublished - 01 Sep 2012

Keywords

  • FOODBORNE DISEASE OUTBREAKS
  • FOOD
  • INFECTIONS
  • TYPEABILITY
  • FIELD GEL-ELECTROPHORESIS
  • SHIGELLA
  • POWDERED INFANT FORMULA
  • PREVALENCE
  • ENTEROBACTER-SAKAZAKII
  • PCR

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Brengi, S. P., O'Brien, S. B., Pichel, M., Iversen, C., Arduino, M., Binsztein, N., Jensen, B., Pagotto, F., Ribot, E. M., Stephan, R., Cernela, N., Cooper, K., & Fanning, S. (2012). Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species. Foodborne pathogens and disease, 9(9), 861-867. https://doi.org/10.1089/fpd.2012.1161