Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species

Silvina P. Brengi; Stephen B. O'Brien; Mariana Pichel; Carol Iversen; Matthew Arduino; Norma Binsztein; Bette Jensen; Franco Pagotto; Efrain M. Ribot; Roger Stephan; Nicole Cernela; Kara Cooper; Seamus Fanning

doi:10.1089/fpd.2012.1161

Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species

Silvina P. Brengi, Stephen B. O'Brien, Mariana Pichel, Carol Iversen, Matthew Arduino, Norma Binsztein, Bette Jensen, Franco Pagotto, Efrain M. Ribot, Roger Stephan, Nicole Cernela, Kara Cooper, Seamus Fanning^*

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

Original language	English
Pages (from-to)	861-867
Number of pages	7
Journal	Foodborne Pathogens and Disease
Volume	9
Issue number	9
DOIs	https://doi.org/10.1089/fpd.2012.1161
Publication status	Published - 01 Sept 2012

Keywords

FOODBORNE DISEASE OUTBREAKS
FOOD
INFECTIONS
TYPEABILITY
FIELD GEL-ELECTROPHORESIS
SHIGELLA
POWDERED INFANT FORMULA
PREVALENCE
ENTEROBACTER-SAKAZAKII
PCR

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology
Food Science
Animal Science and Zoology

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10.1089/fpd.2012.1161

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Brengi, S. P., O'Brien, S. B., Pichel, M., Iversen, C., Arduino, M., Binsztein, N., Jensen, B., Pagotto, F., Ribot, E. M., Stephan, R., Cernela, N., Cooper, K., & Fanning, S. (2012). Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species. Foodborne Pathogens and Disease, 9(9), 861-867. https://doi.org/10.1089/fpd.2012.1161

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title = "Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species",

abstract = "Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.",

keywords = "FOODBORNE DISEASE OUTBREAKS, FOOD, INFECTIONS, TYPEABILITY, FIELD GEL-ELECTROPHORESIS, SHIGELLA, POWDERED INFANT FORMULA, PREVALENCE, ENTEROBACTER-SAKAZAKII, PCR",

author = "Brengi, {Silvina P.} and O'Brien, {Stephen B.} and Mariana Pichel and Carol Iversen and Matthew Arduino and Norma Binsztein and Bette Jensen and Franco Pagotto and Ribot, {Efrain M.} and Roger Stephan and Nicole Cernela and Kara Cooper and Seamus Fanning",

year = "2012",

month = sep,

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doi = "10.1089/fpd.2012.1161",

language = "English",

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pages = "861--867",

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Brengi, SP, O'Brien, SB, Pichel, M, Iversen, C, Arduino, M, Binsztein, N, Jensen, B, Pagotto, F, Ribot, EM, Stephan, R, Cernela, N, Cooper, K & Fanning, S 2012, 'Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species', Foodborne Pathogens and Disease, vol. 9, no. 9, pp. 861-867. https://doi.org/10.1089/fpd.2012.1161

TY - JOUR

T1 - Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species

AU - Brengi, Silvina P.

AU - O'Brien, Stephen B.

AU - Pichel, Mariana

AU - Iversen, Carol

AU - Arduino, Matthew

AU - Binsztein, Norma

AU - Jensen, Bette

AU - Pagotto, Franco

AU - Ribot, Efrain M.

AU - Stephan, Roger

AU - Cernela, Nicole

AU - Cooper, Kara

AU - Fanning, Seamus

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

AB - Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

KW - FOODBORNE DISEASE OUTBREAKS

KW - FOOD

KW - INFECTIONS

KW - TYPEABILITY

KW - FIELD GEL-ELECTROPHORESIS

KW - SHIGELLA

KW - POWDERED INFANT FORMULA

KW - PREVALENCE

KW - ENTEROBACTER-SAKAZAKII

KW - PCR

U2 - 10.1089/fpd.2012.1161

DO - 10.1089/fpd.2012.1161

M3 - Article

SN - 1535-3141

VL - 9

SP - 861

EP - 867

JO - Foodborne Pathogens and Disease

JF - Foodborne Pathogens and Disease

IS - 9

ER -

Development and Validation of a PulseNet Standardized Protocol for Subtyping Isolates of Cronobacter Species

Abstract

Keywords

ASJC Scopus subject areas

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