Development and validation of an HPLC method for the determination of spironolactone and its metabolites in paediatric plasma samples

J.M. Sandall, Paul Collier, Jeffrey Millership, James McElnay

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Abstract An HPLC method has been developed and validated for the determination of spironolactone, 7a-thiomethylspirolactone and canrenone in paediatric plasma samples. The method utilises 200 µl of plasma and sample preparation involves protein precipitation followed by Solid Phase Extraction (SPE). Determination of standard curves of peak height ratio (PHR) against concentration was performed by weighted least squares linear regression using a weighting factor of 1/concentration2. The developed method was found to be linear over concentration ranges of 30–1000 ng/ml for spironolactone and 25–1000 ng/ml for 7a-thiomethylspirolactone and canrenone. The lower limit of quantification for spironolactone, 7a-thiomethylspirolactone and canrenone were calculated as 28, 20 and 25 ng/ml, respectively. The method was shown to be applicable to the determination of spironolactone, 7a-thiomethylspirolactone and canrenone in paediatric plasma samples and also plasma from healthy human volunteers.
Original languageEnglish
Pages (from-to)36-44
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume839(1-2)
Issue number1-2
DOIs
Publication statusPublished - 24 Jul 2006

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Development and validation of an HPLC method for the determination of spironolactone and its metabolites in paediatric plasma samples'. Together they form a unique fingerprint.

  • Cite this