Development and validation of an optical SPR biosensor assay for tiamulin in grass and groundwater

S. Wilson, Terence Fodey, I. Traynor, Christopher Elliott

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Tiamulin (TIA) is an antimicrobial veterinary drug administered subtherapeutically to prevent swine dysentery and pneumonia. Due to its stability, crystalline structure, and water-soluble properties, TIA is a prime candidate for environmental monitoring. However, there are currently no screening methods available for TIA in environmental matrices, such as grass or ground water. In this paper, the development and validation of a screening method using optical SPR biosensor technology is presented. A solvent extraction was carried out on samples prior to analysis using the Biacore Q instrument. The limit of detection for the assay in grass and ground water was 10.8 ng/g and 2.4 ng/ml, respectively. In addition, the assay was shown to be of an acceptable standard with regard to both accuracy and reproducibility.
Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalFOOD AND AGRICULTURAL IMMUNOLOGY
Volume18
Issue number3-4
DOIs
Publication statusPublished - Sep 2007

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)
  • Immunology
  • Toxicology
  • Health, Toxicology and Mutagenesis

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