Development of a bacterial nanoparticle vaccine against Escherichia coli

Melibea Berzosa , Yadira Pastor, Carlos Gamazo*, Juan M Irache

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

2 Citations (Scopus)

Abstract

Currently, different subunit-based vaccine strategies against enterobacteria are being investigated. Among those, bacterial outer membrane vesicles (OMV) are promising candidates because of their immunogenic properties and safety. In order to develop an effective vaccine against this kind of pathogens, it is important to induce both systemic and mucosal immunity. For that reason, the oral route of administration would be an adequate option; although it still represents a challenge due to the particular and harsh conditions of the gut. To overcome these inconveniences, different strategies have been proposed, including the use of polymeric nanoparticles based on the copolymer between methyl vinyl ether and maleic anhydride (Gantrez AN). In the present work, a simple procedure for the preparation of heat-induced OMV (named as HT) obtained from Enterotoxigenic Escherichia coli (ETEC) loaded into these poly(anhydride) nanoparticles is described.
Original languageEnglish
Title of host publicationVaccine design. Methods and protocols, Volume 1. Vaccines for human diseases
EditorsSunil Thomas
PublisherSpringer
Pages345-355
Volume1
ISBN (Electronic)9781071618844
ISBN (Print)9781071618868
DOIs
Publication statusPublished - 17 Dec 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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